New targets of Arabidopsis thioredoxins revealed by proteomic analysis

Autor: Yves Meyer, Christophe H. Marchand, Pierre Le Maréchal, Emmanuelle Issakidis-Bourguet, Myroslawa Miginiac-Maslow, Paulette Decottignies
Přispěvatelé: Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2004
Předmět:
Proteomics
MESH: Oxidation-Reduction
0106 biological sciences
animal structures
Proteome
Molecular Sequence Data
Arabidopsis
MESH: Arabidopsis Proteins
Biology
Reductase
medicine.disease_cause
01 natural sciences
Biochemistry
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Thioredoxins
Botany
medicine
Arabidopsis thaliana
Electrophoresis
Gel
Two-Dimensional
MESH: Arabidopsis
Molecular Biology
Amino acid synthesis
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
MESH: Molecular Sequence Data
MESH: Thioredoxin
Arabidopsis Proteins
MESH: Proteomics
food and beverages
Metabolism
MESH: Electrophoresis
Gel
Two-Dimensional
biology.organism_classification
Plant Leaves
MESH: Plant Leaves
MESH: Proteome
MESH: Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
chemistry
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Thioredoxin
Oxidation-Reduction
Oxidative stress
010606 plant biology & botany
Zdroj: Proteomics
Proteomics, Wiley-VCH Verlag, 2004, 4 (9), pp.2696-706. ⟨10.1002/pmic.200400805⟩
ISSN: 1615-9861
1615-9853
Popis: Proteomics was used to search for putative thioredoxin (TRX) targets in leaves of the model plant, Arabidopsis thaliana. About forty different proteins have been found to be reduced by TRX, after TRX itself has been specifically reduced by its NADPH-dependent reductase. Twenty-one of the identified proteins were already known or recently proposed to be TRX-dependent and nineteen of the proteins were new potential targets. The identified proteins are involved in a wide variety of processes, including the Calvin cycle, metabolism, photosynthesis, folding, defense against oxidative stress and amino acid synthesis. Two proteins from the glycine cleavage complex were also identified as putative TRX targets, and a new role can be postulated in leaves for TRX in defense against herbivores and/or pathogens.
Databáze: OpenAIRE
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