Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential
| Autor: | Kerstin Stingl, Carlus Deneke, Burkhard Malorny, Torsten Semmler, Felix Hartkopf, Julia C. Golz, Maria Borowiak, Lennard Epping, Marie-Theres Knüver |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology lcsh:Medicine Campylobacter coli Biology medicine.disease_cause Genome Article Campylobacter jejuni 03 medical and health sciences Campylobacter Infections medicine Animals Humans ddc:610 Typing lcsh:Science Gene Bacterial genomics Recombination Genetic Whole genome sequencing Genetics Multidisciplinary Whole Genome Sequencing Campylobacter lcsh:R Genetic Variation Food microbiology Gastroenteritis Housekeeping gene 030104 developmental biology Horizontal gene transfer Multilocus sequence typing lcsh:Q Pathogens 610 Medizin und Gesundheit Genome Bacterial |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-60320-y |
| Popis: | Campylobacter is the major bacterial agent of human gastroenteritis worldwide and represents a crucial global public health burden. Species differentiation of C. jejuni and C. coli and phylogenetic analysis is challenged by inter-species horizontal gene transfer. Routine real-time PCR on more than 4000 C. jejuni and C. coli field strains identified isolates with ambiguous PCR results for species differentiation, in particular, from the isolation source eggs. K-mer analysis of whole genome sequencing data indicated the presence of C. coli hybrid strains with huge amounts of C. jejuni introgression. Recombination events were distributed over the whole chromosome. MLST typing was impaired, since C. jejuni sequences were also found in six of the seven housekeeping genes. cgMLST suggested that the strains were phylogenetically unrelated. Intriguingly, the strains shared a stress response set of C. jejuni variant genes, with proposed roles in oxidative, osmotic and general stress defence, chromosome maintenance and repair, membrane transport, cell wall and capsular biosynthesis and chemotaxis. The results have practical impact on routine typing and on the understanding of the functional adaption to harsh environments, enabling successful spreading and persistence of Campylobacter. |
| Databáze: | OpenAIRE |
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