Effects of acute and chronic stress on telencephalic neurochemistry and gene expression in rainbow trout (Oncorhynchus mykiss)
| Autor: | Per Ove Thörnqvist, Danielle Caroline Laursen, Madelene Åberg Andersson, Svante Winberg, Erik Höglund, Maria Moltesen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Telencephalon
0301 basic medicine Serotonin medicine.medical_specialty endocrine system Hydrocortisone Physiology 5-HT Biology Aquatic Science Real-Time Polymerase Chain Reaction Cortisol 03 medical and health sciences Mineralocorticoid receptor Glucocorticoid receptor HPI axis Stress Physiological Internal medicine medicine Animals Chronic stress Receptor Molecular Biology 5-HT receptor Ecology Evolution Behavior and Systematics Medicine (all) Neurochemistry Allostatic load 030104 developmental biology Endocrinology Gene Expression Regulation Oncorhynchus mykiss Insect Science Forebrain Animal Science and Zoology Gene expression |
| Zdroj: | Moltesen, M, Laursen, D C, Thörnqvist, P O, Åberg Andersson, M, Winberg, S & Höglund, E 2016, ' Effects of acute and chronic stress on telencephalic neurochemistry and gene expression in rainbow trout (Oncorhynchus mykiss) ', Journal of Experimental Biology, vol. 219, no. 24, pp. 3907-3914 . https://doi.org/10.1242/jeb.139857 |
| Popis: | By filtering relevant sensory inputs and initiating stress responses, the brain is an essential organ in stress coping and adaptation. However, exposure to chronic or repeated stress can lead to allostatic overload, where neuroendocrinal and behavioral reactions to stress become maladaptive. This work examines forebrain mechanisms involved in allostatic processes in teleost fishes. Plasma cortisol, forebrain serotonergic (5-HTergic) neurochemistry, and mRNA levels of corticotropin-releasing factor (CRF), CRF-binding protein (CRFBP), CRF receptors (CRFR1 and CRFR2), mineralocorticoid receptor (MR), glucocorticoid receptors (GR1 and GR2) and serotonin type 1A (5-HT1A) receptors (5-HT1Aα and 5-HT1Aβ) were investigated at 1 h before and 0, 1 and 4 h after acute stress, in two groups of rainbow trout held in densities of 25 and 140 kg m-3 for 28 days. Generally, being held at 140 kg m-3 resulted in a less pronounced cortisol response. This effect was also reflected in lower forebrain 5-HTergic turnover, but not in mRNA levels in any of the investigated genes. This lends further support to reports that allostatic load causes fish to be incapable of mounting a proper cortisol response to an acute stressor, and suggests that changes in forebrain 5-HT metabolism are involved in allostatic processes in fish. Independent of rearing densities, mRNA levels of 5-HT1Aα and MR were downregulated 4 h post-stress compared with values 1 h post-stress, suggesting that these receptors are under feedback control and take part in the downregulation of the hypothalamic-pituitary-interrenal (HPI) axis after exposure to an acute stressor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|