Characteristics of polyarthritis in rabbits by hyperimmunization with attenuatedEnterococcus faecalis
| Autor: | Hiroaki Yamamoto, Masaki Sekiya, Yasuhiko Konishi, Minoru Kohno, Toshihiko Kohno, Satoshi Mineo |
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| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
Pathology medicine.medical_specialty Knee Joint Arthritis Vaccines Attenuated Toxicology Enterococcus faecalis Pathology and Forensic Medicine Hyperimmunization Pannus Formation Rheumatoid Factor Synovial Fluid medicine Animals Edema Antigens Bacterial biology business.industry Synovial Membrane Cell Biology General Medicine medicine.disease biology.organism_classification C-Reactive Protein Synovial Cell Agglutinins Rheumatoid arthritis Female Immunization Polyarthritis Rabbits business |
| Zdroj: | Experimental and Toxicologic Pathology. 52:247-255 |
| ISSN: | 0940-2993 |
| Popis: | To produce polyarthritis and rheumatoid factor like substance (RFLS), rabbits were hyperimmunized intravenously with 0.02% thimelosal (TMS)-treated Enterococcus faecalis (E. faecalis) as a persistent bacterial flora. Swelling of knee joints occurred at a rate of 41% (27/66), and of shoulder joints at a rate of 25% (17/66) while that of elbow joints occurred at a rate of 4.5% (3/66). On culturing of knee joint fluids, no colonies appeared while 2/4 fluid specimens from the shoulder joints gave positive colonies for 78 days after the first immunization; thereafter, no colonies appeared. On histological examination, in early stages, acute inflammatory reactions with degenerative changes of synovial tissue was observed. In later stages, chronic inflammatory changes, proliferation of synovial cells with pannus formation, destruction of articular cartilage and subchondral bone were observed. RFLS titer showed bi-phasic peaks at 11 days and 41 days after the first immunization. A high incidence of polyarthritis, particularly knee joints, occurred. Thus, hyperimmunization with attenuated E. faecalis as a normal intestinal flora may provide an animal model of chronic polyarthritis. |
| Databáze: | OpenAIRE |
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