Combined acupuncture and HuangDiSan treatment affects behavior and synaptophysin levels in the hippocampus of senescence-accelerated mouse prone 8 after neural stem cell transplantation
Autor: | Hui-Yan Shi, Jian-Wei Liu, Jianchun Yu, Jiang-Wei Shi, Zhen Li, Bo-Hong Kan, Chunlei Zhou, Lan Zhao, Jingxian Han |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
synaptophysin Hippocampus neurons Pharmacology Hippocampal formation Zusanli 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Acupuncture Medicine nerve regeneration biology Chinese medicine business.industry behavior Dentate gyrus neurodegeneration Alzheimer's disease microenvironment Neural stem cell Transplantation 030104 developmental biology Synaptophysin biology.protein neural stem cell transplantation business neural regeneration 030217 neurology & neurosurgery Research Article |
Zdroj: | Neural Regeneration Research |
ISSN: | 1876-7958 1673-5374 |
Popis: | Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 (SAMP8) mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer's disease model mice. Sanjiao acupuncture was used to stimulate Danzhong (CV17), Zhongwan (CV12), Qihai (CV6), bilateral Xuehai (SP10) and bilateral Zusanli (ST36) 15 days before and after implantation of neural stem cells (5 × 105) into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica, was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone, learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression. |
Databáze: | OpenAIRE |
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