Protective effects of minocycline on experimental spinal cord injury in rats
| Autor: | Sedat Motor, Recep Dokuyucu, Atilla Yilmaz, Nebi Yilmaz, Mustafa Aras, Yurdal Seraslan, Erkin Ozgiray, Murat Altaş |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Minocycline Antioxidants Sham group Superoxide dismutase chemistry.chemical_compound Malondialdehyde Internal medicine medicine Animals Rats Wistar Spinal cord injury Spinal Cord Injuries General Environmental Science chemistry.chemical_classification biology business.industry Glutathione peroxidase Free Radical Scavengers medicine.disease Rats Disease Models Animal Oxidative Stress Antioxidant capacity Neuroprotective Agents Endocrinology Spinal Cord chemistry Anesthesia biology.protein General Earth and Planetary Sciences business medicine.drug |
| Zdroj: | Injury. 46:1471-1474 |
| ISSN: | 0020-1383 |
| DOI: | 10.1016/j.injury.2015.05.018 |
| Popis: | The effects of minocycline on neuronal injury after spinal cord injury (SCI) are limited and controversial. Therefore we aimed to investigate the protective effects of minocycline on tissue and on serum concentrations of malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, tissue total antioxidant and oxidant status (TAS and TOS, respectively), and AST and LDH levels in rats with SCI.This study was performed on 7-8 weeks 38 male Wistar albino rats. The animals were randomly divided into five groups: group 1, Sham (n=8); group 2, SCI (spinal cord injury)/control (n=8); group 3, SCI+minocycline3 (n=7); group 4, SCI+minocycline30 (n=8) and group 5 SCI+minocycline90 (n=7). Blood and tissue samples were analysed for MDA, SOD, GSH-Px, TAS, TOS, AST and LDH levels.The MDA levels were significantly higher in SCI group compared to sham group (p0.001), and MDA levels were also significantly higher in SCI group compared to SCI+M3, SCI+M30, SCI+M90 (p0.05). SOD levels were significantly higher in SCI+M30 when compared to SCI and SCI+M3 groups (p0.05). GSH-Px levels decreased significantly in SCI and SCI+M3 groups compared to sham (p0.05). SCI+M3 group showed significantly decreased levels of TAS and TOS compared to SCI group (p0.05). TAS and TOS levels significantly increased in SCI+M90 group compared to SCI+M3 and SCI+M30 groups (p0.05).The present study demonstrates the dose-dependent antioxidant activity of minocycline against spinal cord injury in rats. Minocycline administration increased antioxidant enzyme levels and improved total antioxidant status. |
| Databáze: | OpenAIRE |
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