Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma
| Autor: | Ruizhen Bai, Yuan Weng, Zhi-qiang Wang, Huan Zhou, Linfang Jin, Ming Cai, Jiru Zhang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Lung adenocarcinoma
Male Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Lung Neoplasms Physiology Carcinogenesis Cell Molecular Sequence Data Adenocarcinoma of Lung Biology ADAM17 Protein Adenocarcinoma lcsh:Physiology lcsh:Biochemistry Invasion Western blot Cell Movement Genes Reporter Cell Line Tumor medicine Gene silencing Humans lcsh:QD415-436 Luciferases Aged ADAM17 Metalloproteinase lcsh:QP1-981 medicine.diagnostic_test Base Sequence EMT miR-326 Middle Aged Oligonucleotides Antisense medicine.disease In vitro Gene Expression Regulation Neoplastic ADAM Proteins MicroRNAs medicine.anatomical_structure Cell culture Cancer cell Cancer research Diffusion Chambers Culture Female Signal Transduction |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 36, Iss 3, Pp 1175-1185 (2015) |
| ISSN: | 1421-9778 |
| Popis: | Background/Aims: A disintegrin and metalloprotease (ADAM) 17 has been reported to be implicated in cancer cells invasion. Nevertheless, its potential role in lung adenocarcinoma has not been addressed clearly. Methods: RT-PCR and Western blot were used to detect the expression of miR-326 and ADAM17 in lung adenocarcinoma samples (n=73). miR-326 mimics and inhibitor were tansfected in human A549 and SPCA1 cell lines. The transwell assay was used to detect the cell invasive ability. The regulation mechanism was evaluated by luciferase reporter assay. The markers of (epithelial-to-mesenchymal transition) EMT were detected by using Western blot assay. Results: We found increased expression of ADAM17 in lung adenocarcinoma and cell lines. In vitro, up-regulation of ADAM17 promoted cells invasion, while silencing of ADAM17 inhibited cells invasion. Meanwhile, ADAM17 could affect the markers of EMT. Furthermore, we confirmed that ADAM17 is a target of miR-326, which is involved in EMT and cells invasion. Conclusions: These findings revealed that ADAM17, a target of miR-326, promoted EMT-induced cells invasion in lung adenocarcinoma. |
| Databáze: | OpenAIRE |
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