Functional and phenotypical alterations of polymorphonuclear cells in Sézary syndrome patients
| Autor: | Chiara Merlino, Matteo Brizio, Pietro Quaglino, Annamaria Cuffini, Daniela Scalas, Valeria Allizond, Giuliana Banche, Maria Teresa Fierro, Alessandra Comessatti, Mauro Novelli, Maria Grazia Bernengo |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Neutrophils Phagocytosis Dermatology medicine Humans Sezary Syndrome Prospective Studies Receptor Aged Aged 80 and over Innate immune system biology business.industry Toll-Like Receptors Middle Aged Flow Cytometry medicine.disease Lymphoma Klebsiella pneumoniae Toll-Like Receptor 5 TLR2 Phenotype Integrin alpha M TLR5 Immunology biology.protein Female business Intracellular |
| Zdroj: | European Journal of Dermatology. 21:921-929 |
| ISSN: | 1952-4013 1167-1122 |
| DOI: | 10.1684/ejd.2011.1544 |
| Popis: | Sézary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma (CTCL), has a poor prognosis and infections represent the most frequent cause of death. Polymorphonucleate granulocytes (PMNs) constitute an essential part of the innate immune system: their phagocytic and killing activity against pathogens is mediated by the interactions between Toll-like receptors (TLRs) and the Pathogen-associated molecular patterns (PAMPs). The aim of this study was to investigate PMN functional activity and phenotype in SS patients and their correlation with the onset of infectious complications. This prospective study enrolled 18 consecutive SS patients; PMN functional activity was evaluated by phagocytosis and intracellular killing tests towards Klebsiella pneumoniae. Flow-cytometry was applied to analyze PMN phenotype. PMNs from SS patients displayed a reduced phagocytic activity and intracellular killing against K. pneumoniae at 30 min and 60 min, more pronounced in SS patients with recurrent infections. CD11b and CD66b median fluorescence intensity (MFI) was significantly higher in SS than in healthy subjects, whereas CD62L MFI was decreased. No significant differences in TLR2, 4, 8 and 9 percentage expression or MFI were found. An increased TLR5 percentage expression was documented. The impairment in PMN functional activities in SS could favour the immune-suppression and raise infection risk. |
| Databáze: | OpenAIRE |
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