Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats
| Autor: | M. H. Shalavadi, V. M. Chandrashekhar, Iranna S. Muchchandi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Convolvulus Ischemia Pharmacology Convolvulus pluricaulis medicine.disease_cause Neuroprotection Antioxidants Brain Ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine.artery Drug Discovery Animals Medicine Common carotid artery 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug biology Plant Extracts business.industry Cerebral infarction Cerebral Infarction biology.organism_classification medicine.disease Free radical scavenger Rats Stroke Oxidative Stress Neuroprotective Agents Reperfusion Injury 030220 oncology & carcinogenesis Female Quercetin business Microtubule-Associated Proteins Reperfusion injury Oxidative stress |
| Zdroj: | Journal of Ethnopharmacology. 249:112393 |
| ISSN: | 0378-8741 |
| Popis: | Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found.To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury.The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200-250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out.The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p 0.001) and an increase in superoxide dismutase (p 0.01, p 0.001), catalase (p 0.01, p 0.001), glutathione (p 0.001), and total thiol (p 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract.Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. |
| Databáze: | OpenAIRE |
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