Consequential Apoptosis in the Cerebellum Following Injury to the Developing Rat Forebrain
Autor: | Catherine Sarraf, Huseyin Mehmet, Umesh Joashi, A. David Edwards, Deanna L. Taylor |
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Rok vydání: | 2006 |
Předmět: |
Programmed cell death
Cerebellum Pathology medicine.medical_specialty Blotting Western Purkinje cell Apoptosis Biology Cell morphology Pathology and Forensic Medicine Prosencephalon Microscopy Electron Transmission medicine Animals Rats Wistar Diaschisis In Situ Hybridization Research Articles Neurons General Neuroscience Hypoxia (medical) Rats medicine.anatomical_structure nervous system Hypoxia-Ischemia Brain Forebrain Neurology (clinical) Poly(ADP-ribose) Polymerases medicine.symptom Neuroscience |
Zdroj: | Brain Pathol |
ISSN: | 1750-3639 1015-6305 |
Popis: | In focal brain lesions, alterations in blood flow and cerebral metabolism can be detected in brain areas remote from the primary injury. The cellular consequences of this phenomenon, originally termed diaschisis, are not fully understood. Here, we report that in two distinct models of forebrain injury, neuronal death in the cerebellum, a site distant to the primary injury, results as consequence of neuronal loss in the forebrain. Fourteen‐day‐old rats were subjected to unilateral forebrain injury, achieved by either hypoxia‐ischemia (right carotid artery ligation and hypoxia) or direct needle injury to brain tissue. At defined times after injury, the presence of apoptosis was investigated by cell morphology, in situ end labeling, electron microscopy and poly‐ADP‐ribose polymerase (PARP) cleavage. Injury to the rat forebrain following hypoxia‐ischemia increased apoptosis in the internal granular and Purkinje cell layers of the cerebellum, a site distant to that of the primary injury. The number of apoptotic cells in the cerebellum was significantly related to cell death in the hippocampus. Similarly, direct needle injury to the forebrain resulted in extensive apoptotic cell death in the cerebellum. These results emphasize the intimate relationship between defined neuronal populations in relatively distant brain areas and suggest a cellular basis for diaschisis. |
Databáze: | OpenAIRE |
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