Hepatic extraction and hepatic action of insulin, glucagon, and epinephrine in bivascularly perfused rat liver
| Autor: | Hiroshi Ochi, Tazue Hoshino, Tadasu Ikeda, Hiroto Mashiba, Yasushi Tanaka, Katsumi Fujiyama, Hikari Terasawa, Masahiko Ishimura |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Epinephrine Physiology medicine.medical_treatment Carbohydrate metabolism Glucagon Oxygen Consumption Celiac artery medicine.artery Internal medicine medicine Animals Insulin Rats Wistar business.industry General Medicine Rats Perfusion Endocrinology medicine.anatomical_structure Glucose Liver Rat liver business Artery medicine.drug |
| Zdroj: | The Japanese journal of physiology. 43(3) |
| ISSN: | 0021-521X |
| Popis: | Using a bivascularly perfused rat liver, we investigated the hepatic extraction and hepatic action on glucose output of insulin, glucagon, and epinephrine. The liver was perfused for 70 min without recirculation via the portal vein and hepatic artery (3.0 ml/(min.g liver) from portal vein and 1.0 ml/(min.g liver) from celiac artery). Low and high concentration of insulin, glucagon, or epinephrine (450 and 4,500 pM, 21.5 and 215 pM, or 410 and 4,100 pM, respectively) was added to the perfusing medium via portal vein (P-liver), via celiac artery (H-liver), or via portal vein and celiac artery (H-P-liver). Hepatic extraction of insulin, glucagon, or epinephrine was not significantly different among H- (56 +/- 9 and 21 +/- 5%, 31 +/- 7 and 24 +/- 6%, or 22 +/- 8 and 17 +/- 5%), P- (50 +/- 10 and 23 +/- 6%, 32 +/- 9 and 23 +/- 7%, or 22 +/- 9 and 18 +/- 6%), and H-P-liver (55 +/- 11 and 24 +/- 6%, 30 +/- 9 and 26 +/- 8%, or 20 +/- 9 and 17 +/- 6%). Glucagon- or epinephrine-induced increase in glucose output was also similar in H- (245 +/- 47 and 498 +/- 82 mumol/30 min, or 112 +/- 21 and 215 +/- 38 mumol/30 min), P- (258 +/- 51 and 512 +/- 95 mumol/30 min, or 128 +/- 20 and 220 +/- 36 mumol/30 min), and H-P-liver (263 +/- 58 and 515 +/- 94 mumol/30 min, or 129 +/- 22 and 225 +/- 39 mumol/30 min).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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