Oral administration of lipopolysaccharides from Escherichia coli (serotype O111:B4) does not induce an effective systemic immune response in milk-fed Holstein calves
| Autor: | Lorenzo E. Hernández-Castellano, M.B. Samarasinghe, Jakob Sehested, Torben Larsen |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Male Serotype medicine.medical_specialty Administration Oral Immunoglobulins Serogroup calves 03 medical and health sciences Immune system Oral administration Internal medicine Escherichia coli Genetics medicine Animals Serum amyloid A 030304 developmental biology 0303 health sciences biology business.industry Body Weight Haptoglobin 0402 animal and dairy science Acute-phase protein Albumin 04 agricultural and veterinary sciences lipopolysaccharides 040201 dairy & animal science Immunity Innate immune system Milk Endocrinology biology.protein Cattle lipids (amino acids peptides and proteins) Animal Science and Zoology Antibody business Acute-Phase Proteins Food Science |
| Zdroj: | Samarasinghe, M B, Sehested, J, Larsen, T & Hernández-Castellano, L E 2020, ' Oral administration of lipopolysaccharides from Escherichia coli (serotype O111:B4) does not induce an effective systemic immune response in milk-fed Holstein calves ', Journal of Dairy Science, vol. 103, no. 6, pp. 5525-5531 . https://doi.org/10.3168/jds.2019-17404 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2019-17404 |
| Popis: | It is well established that intravenous administration of lipopolysaccharides (LPS)—cell wall components from gram-negative bacteria—induce acute inflammatory responses in dairy calves, but the effect of oral administration of LPS to dairy calves is currently unknown. To evaluate the effects of oral administration of LPS derived from Escherichia coli (serotype O111:B4) on innate immune responses in milk-fed Holstein calves, 20 visually healthy calves (34 ± 1 d) received 4 L of milk with LPS (12 μg/kg body weight; n = 10; LPS) or without LPS (n = 10; control) at the morning feeding. Samples were collected at 0.5 h before the morning feeding and at 3, 6, 24, 48, 72, and 168 h after the morning feeding to measure rectal temperature and heart rate, as well as plasma-negative and plasma-positive acute phase proteins (i.e., haptoglobin, serum amyloid A, albumin, total protein, and fibrinogen) and immunoglobulin concentrations (IgG, IgM, and IgA). None of these measurements was affected by the oral administration of LPS. Oral administration of LPS at 12 μg/kg of body weight did not induce an acute inflammatory response in visually healthy milk-fed Holstein calves when administered in milk. |
| Databáze: | OpenAIRE |
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