Prion early kinetics revisited using a streptomycin-based PrPres extraction method
| Autor: | Edwige Leclere, Hervé Perron, Anna Bencsik, Anthony W. Coleman, Latifa Chouaf-Lakhdar, Jean-Marie Seigneurin, Aly Moussa |
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| Rok vydání: | 2008 |
| Předmět: |
Prions
animal diseases Biophysics Scrapie Spleen Biology Biochemistry Prion Diseases Mice Western blot Methods medicine Animals Chemical Precipitation Prion protein Molecular Biology Brain Chemistry medicine.diagnostic_test Cell Biology Virology nervous system diseases Mice Inbred C57BL Kinetics medicine.anatomical_structure Streptomycin Immunohistochemistry Cattle Female Extraction methods Infectious agent medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 372:429-433 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.05.040 |
| Popis: | The use of streptomycin in the PrP sc detection procedures represents a new and attractive way to detect more PrP sc , the best marker for the transmissible spongiform encephalopathies (TSEs). Actually, the streptomycin PrP sc aggregating property reported recently was established as beneficial in PrP sc detection using immunohistochemistry in diagnostic as well as in experimental conditions. The present study reports in details how to use advantageously this original streptomycin property in PrP res biochemical extraction and detection. Using TSE diagnostic brain material, specificity and increased sensitivity using streptomycin-treated samples were substantiated. Then an early sequential brain and spleen sampling (from 7 to 49 days post-inoculation) from C57Bl/6 mice inoculated intra-cerebrally or intra-peritoneally with C506M3 scrapie strain was analysed using streptomycin versus ultracentrifugation PrP res extraction. Whatever the inoculation route, streptomycin allowed earlier PrP res detection in spleen (7 d.p.i.), then in brain suggesting a stronger affinity of the infectious agent for the lymphoid compartment. |
| Databáze: | OpenAIRE |
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