Comprehensive comparison of polysaccharides from Ganoderma lucidum and G. sinense: chemical, antitumor, immunomodulating and gut-microbiota modulatory properties
| Autor: | Li-Feng Li, Aiping Lu, Ji-Xia Zhang, Quan-Bin Han, Zhi-Peng Li, Tin-Long Wong, Hong-Bing Liu, Su-Ping Bai, Quan-Wei Zhang, Hau-Yee Fung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Reishi Cell Survival Phagocytosis lcsh:Medicine Antineoplastic Agents 02 engineering and technology Polysaccharide Article Terpene Immunomodulation 03 medical and health sciences Mice Cell Movement Cell Line Tumor Animals lcsh:Science Sugar chemistry.chemical_classification Multidisciplinary Molecular Structure Spectrum Analysis lcsh:R Fungal Polysaccharides 021001 nanoscience & nanotechnology Xenograft Model Antitumor Assays Gastrointestinal Microbiome Molecular Weight 030104 developmental biology RAW 264.7 Cells chemistry Biochemistry Cell culture Cancer cell lcsh:Q 0210 nano-technology Function (biology) |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Both Ganoderma lucidum (GL) and G. sinense (GS) are used as Lingzhi in China. Their functions are assumed to mainly derive from triterpenes and polysaccharides; however, the two species have very different triterpenes profiles, if this was the case, then the bioactivity of these two species should differ. Instead, could the polysaccharides be similar, contributing to the shared therapeutic basis? In this study, two main polysaccharide fractions from different batches of GL and GS were systematically compared by a series of chemical and biological experiments. The results showed that the polysaccharides from two species shared the same structural features in terms of mono-/oligo-saccharide profiles, molecular size, sugar linkages, and IR/NMR spectra. In addition, these polysaccharides showed similar tumor-suppressive activity in mice. Further study on RAW264.7 cells indicated that these polysaccharides exhibited similar inducing effects to macrophages, as evaluated in the phagocytosis function, NO/cytokines production, inhibition against the viability and migration of cancer cells. Mechanistic investigation revealed the identical activation via TLR-4 related MAPK/NF-κB signaling pathway and gut-microbiota modulatory effects. In summary, GL and GS polysaccharides presented similar chemical features, antitumor/immunomodulating activities and mechanism; this establishes polysaccharides as the active principles and supports the official use of both species as Lingzhi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|