Tiling array study of MNNG treated Escherichia coli reveals a widespread transcriptional response
| Autor: | Karin Lagesen, Torbjørn Rognes, Alexander D. Rowe, Jessica M. Lindvall, Knut Ivan Kristiansen, Ragnhild Weel-Sneve, James Alexander Booth, Gard O. S. Thomassen, Magnar Bjørås |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Methylnitronitrosoguanidine
animal structures RNA Untranslated genetic structures Transcription Genetic genetic processes Adaptation Biological Biology medicine.disease_cause Article Untranslated Regions Operon medicine Escherichia coli natural sciences Promoter Regions Genetic Genetics Multidisciplinary Tiling array Gene Expression Profiling Reproducibility of Results Gene Expression Regulation Bacterial Mutation Transcriptional response sense organs Transcriptome |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon – consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli following a MNNG challenge. Along with the expected upregulation of the adaptive response genes (ada, alkA and alkB), we identified a number of differentially expressed transcripts, both novel and annotated. This indicates a wider regulatory response than previously documented. There were 250 differentially-expressed and 2275 similarly-expressed unannotated transcripts. We found novel upregulation of several stress-induced transcripts, including the SOS inducible genes recN and tisAB, indicating a novel role for these genes in alkylation repair. Furthermore, the ada-regulon A and B boxes were found to be insufficient to explain the regulation of the adaptive response genes after MNNG exposure, suggesting that additional regulatory elements must be involved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|