The Role of Melanotransferrin (CD228) in the regulation of the differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells (hBM-MSC)
| Autor: | Sooho Lee, Maria Dubon, Dong-Chul Kang, Jae-Yong Lee, Ji-Hong Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Osteocalcin
Cell Line osteogenesis adipogenesis 03 medical and health sciences 0302 clinical medicine Humans Homeodomain Proteins Gene knockdown mesenchymal stem cells Membrane Glycoproteins biology Chemistry Mesenchymal stem cell Gene Expression Regulation Developmental Cell Differentiation General Medicine differentiation Fibroblasts Embryo Mammalian Embryonic stem cell Cell biology RUNX2 Melanotransferrin cell surface markers Sp7 Transcription Factor Adipogenesis Gene Knockdown Techniques biology.protein Alkaline phosphatase 030211 gastroenterology & hepatology Transcription Factors Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Melanotransferrin (CD228), firstly reported as a melanoma-associated antigen, is a membrane-bound glycoprotein of an iron-binding transferrin homolog. CD228 was found to be expressed significantly higher in human bone marrow-derived mesenchymal stem cells (hBM-MSC) than in human embryonic fibroblasts (FB) by RT-PCR, western blotting and flow cytometry. The expression of CD228 declined in aged hBM-MSC as osteogenesis-related genes did. We examined a possible role for CD228 in the regulation of osteogenesis and adipogenesis of hBM-MSC. Surprisingly, siRNA-mediated CD228 knockdown increased the expression of the transcription factor DLX5 and enhanced osteogenesis of hBM-MSC evidenced by an increased expression of the runt-related transcription factor 2 (RUNX2), osterix (Osx), and osteocalcin (OC), as well as higher alkaline phosphatase (ALP) activity and extracellular calcium deposition. Interestingly, hBM-MSC transfected with CD228 siRNA also showed an increase in intracellular lipid level during adipogenesis, indicated by oil red O staining of differentiated adipocytes. Overall, our study unveils CD228 as a cell surface molecule expressed by young hBM-MSC, but not by FB. It also provides evidence to suggest a role for CD228 as a negative regulator of osteogenesis and of lipid accumulation during adipogenesis in hBM-MSC in vitro. |
| Databáze: | OpenAIRE |
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