A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings
| Autor: | Sarah E. Maddocks, Michele E Barbour, Peter F. Duckworth, Richard S. Rowlands |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chronic wound Staphylococcus aureus Alginates Staphylococcus 030106 microbiology Cell Culture Techniques Colony Count Microbial Biology medicine.disease_cause Microbiology 03 medical and health sciences Flow system Anti-Infective Agents Glucuronic Acid Pseudomonas medicine Pseudomonas Infections Biofilm Hexuronic Acids Wound Chlorhexidine Equipment Design biochemical phenomena metabolism and nutrition Staphylococcal Infections biology.organism_classification Antimicrobial Bandages Wound infection 030104 developmental biology Biofilms Pseudomonas aeruginosa Wound Infection Computer-Aided Design medicine.symptom |
| Zdroj: | Duckworth, P F, Rowlands, R S, Barbour, M E & Maddocks, S E 2018, ' A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings ', Microbiological Research, vol. 215, pp. 141-147 . https://doi.org/10.1016/j.micres.2018.07.009 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2018.07.009 |
| Popis: | Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported. The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings. Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72 h giving consistent log counts of 8–10 colony forming units (CFU). There was a 3–4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48 h, and left in situ for a further 24 h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20 °C and 37 °C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37 °C. |
| Databáze: | OpenAIRE |
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