ЭФФЕКТЫ ДЕЙСТВИЯ ФИКОБИЛИПРОТЕИНОВ ARTHROSPIRA PLATENSIS (NORDSTEDT) GOMONT В РАСТИТЕЛЬНЫХ ТКАНЯХ: АНТИОКСИДАНТНАЯ АКТИВНОСТЬ В ГЛЮКОЗООКСИДАЗНОЙ ТЕСТ-СИСТЕМЕ И РАЗОБЩЕНИЕ ОКИСЛЕНИЯ И ФОСФОРИЛИРОВАНИЯ В МИТОХОНДРИЯХ
| Autor: | Ruslan G. Gevorgiz, I. V. Fedoseeva, Alexey Stepanov, Olga Ivanovna Grabelnykh, Alisa A. Aksenova, Elizaveta Alekseevna Polyakova |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
arthrospira platensis митохондрии Science medicine.medical_treatment с-фикоцианин Oxidative phosphorylation General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Glucose oxidase activity глюкозооксидаза medicine Glucose oxidase пероксид водорода Hydrogen peroxide Allophycocyanin biology Chemistry Phycobiliprotein Agriculture biology.organism_classification Biochemistry biology.protein фикобилипротеины разобщение процессов окисления и фосфорилирования General Agricultural and Biological Sciences Arthrospira |
| Zdroj: | Siberian Journal of Life Sciences and Agriculture, Vol 13, Iss 2, Pp 202-224 (2021) |
| ISSN: | 2658-6657 2658-6649 |
| Popis: | Background. Cyanobacterium Arthrospira (Spirulina) platensis has a unique biochemical composition and is widely used in various fields, including medicine and agriculture. The main phycobiliproteins (PBPs) of spirulina – C-phycocyanin and allophycocyanin protect animal cells from oxidative stress and mitochondrial dysfunction. At the same time there is little information about the antioxidant properties of PBPs in a plant cell and their influence on the bioenergetic parameters of plant mitochondria. Purpose. The aim of this study was to determine the effect of A. platensis PBPs extract on glucose oxidase activity and functioning of potato mitochondria. Materials and methods. PBPs were isolated from the crude biomass of A. platensis (Nordstedt) Gomont (strain IBSS-31) using cold extraction and precipitation with acetone. The protein extract with a high proportion of C-phycocyanin was used at concentrations of 0,025 – 0,25 mg/ml. The antioxidant properties of PBPs were evaluated by the inhibition of the glucose oxidase activity of potato extracts with increased expression of the GOX gene. The oxidative and phosphorylating activity of mitochondria in the presence of PBPs was measured by the polarographic method. Statistical data processing was carried out using SigmaPlot v. 14.0. Results. The glucose oxidase test revealed the antioxidant activity of the PBPs extract, which was pronounced at a concentration of 0,25 mg / ml. At the same concentration, the extract caused uncoupling of oxidation and phosphorylation in potato mitochondria during succinate oxidation, associated with an increase in the state 4 respiration rate and a decrease of the respiratory control coefficient. Conclusion. Thus, phycobiliproteins of A. platensis in a concentration-dependent manner reduce the generation of hydrogen peroxide in the glucose oxidase test system and cause uncoupling of the oxidation and phosphorylation in the plant mitochondria. |
| Databáze: | OpenAIRE |
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