Two genome sequences of the same bacterial strain, Gluconacetobacter diazotrophicus PAl 5, suggest a new standard in genome sequence submission
| Autor: | Eric W. Triplett, Adriana Giongo, Heather L. Tyler, Ursula N. Zipperer |
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| Jazyk: | angličtina |
| Předmět: |
Comparative genomics
Genetics Whole genome sequencing 0303 health sciences 030306 microbiology Optical Mapping White Paper Sequence assembly Hybrid genome assembly Genome project Biology biology.organism_classification Genome Gluconacetobacter 03 medical and health sciences chromosomal rearrangements 030304 developmental biology Sequence (medicine) |
| Zdroj: | Standards in Genomic Sciences |
| ISSN: | 1944-3277 |
| DOI: | 10.4056/sigs.972221 |
| Popis: | Gluconacetobacter diazotrophicus PAl 5 is of agricultural significance due to its ability to provide fixed nitrogen to plants. Consequently, its genome sequence has been eagerly anticipated to enhance understanding of endophytic nitrogen fixation. Two groups have sequenced the PAl 5 genome from the same source (ATCC 49037), though the resulting sequences contain a surprisingly high number of differences. Therefore, an optical map of PAl 5 was constructed in order to determine which genome assembly more closely resembles the chromosomal DNA by aligning each sequence against a physical map of the genome. While one sequence aligned very well, over 98% of the second sequence contained numerous rearrangements. The many differences observed between these two genome sequences could be owing to either assembly errors or rapid evolutionary divergence. The extent of the differences derived from sequence assembly errors could be assessed if the raw sequencing reads were provided by both genome centers at the time of genome sequence submission. Hence, a new genome sequence standard is proposed whereby the investigator supplies the raw reads along with the closed sequence so that the community can make more accurate judgments on whether differences observed in a single stain may be of biological origin or are simply caused by differences in genome assembly procedures. |
| Databáze: | OpenAIRE |
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