Up-regulation of defense enzymes is responsible for low reactive oxygen species in malignant prostate cancer cells
Autor: | Chang Jin Lim, Eun-Hee Park, Hyun Jung Kang, Seunghwan Lim, Wook Jin, Suntaek Hong, Hye Won Lim, Su Jung Kim, Kisup Ahn |
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Rok vydání: | 2005 |
Předmět: |
Male
Thioredoxin-Disulfide Reductase Clinical Biochemistry Glutathione reductase Biology urologic and male genital diseases medicine.disease_cause Biochemistry Antioxidants chemistry.chemical_compound Cell Line Tumor LNCaP medicine Humans neoplasms Molecular Biology chemistry.chemical_classification Reactive oxygen species Prostatic Neoplasms Glutathione Up-Regulation Gene Expression Regulation Neoplastic Oxidative Stress chemistry Cell culture Tumor progression Enzyme Induction Cancer research Molecular Medicine Thioredoxin Reactive Oxygen Species Oxidative stress |
Zdroj: | Experimentalmolecular medicine. 37(5) |
ISSN: | 1226-3613 |
Popis: | Reactive oxygen species (ROS) are involved in a diversity of important phenomena in the process of tumor development. To investigate the alterations of oxidative stress and their related systems in tumor progression, a variety of components in the antioxidative stress defense system were examined in prostate cancer cell lines, PC3 and LNCaP. Cell surface molecules involved in metastasis were expressed highly in PC3 cells compared with LNCaP cells, and strong invasion ability was shown in PC3 cells only. ROS level in LNCaP cells was twice higher than that in PC3 cells, although nitric oxide (NO) level was similar between the two cell lines. The content of GSH increased up to about 2-fold in PC3 compared with LNCaP. Activities of glutathione reductase, thioredoxin reductase, and glutathione S-transferase except catalase are significantly higher in PC3 cells than in LNCaP cells. Furthermore, oxidative stress-inducing agents caused down-regulation of GSH and glutathione S-transferase much more significantly in LNCaP cells than in PC3 cells. These results imply that malignant tumor cells may maintain low ROS content by preserving relatively high anti-oxidative capacity, even in the presence of stressful agents. |
Databáze: | OpenAIRE |
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