Sequential adaptation of perfusion and transport conditions significantly improves vascular construct recellularization and biomechanics
| Autor: | Aurore Van de Walle, Marc C. Moore, Peter S. McFetridge |
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| Rok vydání: | 2019 |
| Předmět: |
Pore size
Scaffold Cell Survival 0206 medical engineering Myocytes Smooth Muscle Biomedical Engineering Medicine (miscellaneous) Lumen (anatomy) Biological Transport Active 02 engineering and technology Article Biomaterials Glycosaminoglycan 03 medical and health sciences Human Umbilical Vein Endothelial Cells Distribution (pharmacology) Humans 030304 developmental biology 0303 health sciences Tissue Engineering Tissue Scaffolds Chemistry Biomechanics Cell migration 020601 biomedical engineering Blood Vessel Prosthesis Extracellular Matrix Perfusion Biomedical engineering |
| Zdroj: | J Tissue Eng Regen Med |
| ISSN: | 1932-7005 |
| Popis: | Recellularization of ex vivo-derived scaffolds remains a significant hurdle primarily due to the scaffolds subcellular pore size that restricts initial cell seeding to the scaffolds periphery and inhibits migration over time. With the aim to improve cell migration, repopulation, and graft mechanics, the effects of a four-step culture approach were assessed. Using an ex vivo-derived vein as a model scaffold, human smooth muscle cells were first seeded onto its ablumen (Step 1: 3 hr) and an aggressive 0-100% nutrient gradient (lumenal flow under hypotensive pressure) was created to initiate cell migration across the scaffold (Step 2: Day 0 to 19). The effects of a prolonged aggressive nutrient gradient created by this single lumenal flow was then compared with a dual flow (lumenal and ablumenal) in Step 3 (Day 20 to 30). Analyses showed that a single lumenal flow maintained for 30 days resulted in a higher proportion of cells migrating across the scaffold toward the vessel lumen (nutrient source), with improved distribution. In Step 4 (Day 31 to 45), the transition from hypotensive pressure (12/8 mmHg) to normotensive (arterial-like) pressure (120/80 mmHg) was assessed. It demonstrated that recellularized scaffolds exposed to arterial pressures have increased glycosaminoglycan deposition, physiological modulus, and Young's modulus. By using this stepwise conditioning, the challenging recellularization of a vein-based scaffold and its positive remodeling toward arterial biomechanics were obtained. |
| Databáze: | OpenAIRE |
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