The binding of lac repressor and the catabolite gene activator protein to halogen-substituted analogues of poly[d(A-T)]
| Autor: | Arthur D. Riggs, Syr-Yaung Lin |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
inorganic chemicals
Stereochemistry Catabolite repression Lactose Biology Lac repressor Binding Competitive Biochemistry Genetics and Molecular Biology (miscellaneous) Structure-Activity Relationship chemistry.chemical_compound Polydeoxyribonucleotides Bacterial Proteins Escherichia coli medicine Gene Binding Sites Kinetics Genes chemistry Mechanism of action Biochemistry Halogen biology.protein medicine.symptom DNA Protein Binding Catabolite activator protein |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 432:185-191 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(76)90160-x |
| Popis: | We have measured the binding of two regulatory proteins to the complete halogen-substituted series of poly[d(A-T)] analogues. Both the lac repressor and the catabolite gene activator protein were found to bind more strongly to all of the halogen-substituted DNAs than they do to poly[d(A-T)]. For both proteins, the order of binding preference is poly[d(A-ioU)] is greater than poly[d(A-brU)] is greater than or equal to poly[d(A-clU)] is greater than poly[d(A-flU)] is greater than poly[d(A-T)]. Quantitative data on the binding of these proteins to poly[d(A-U)] is also given. The significance of these results for the mechanism of protein-DNA interaction is discussed. This is the first report that an activator protein binds more strongly to a halogen-substituted DNA; we discuss this result with regard to the mechanism of action of bromodeoxyruidine and other halogen-substituted base analogues on the inhibition and induction of differentiation in eucaryotic cells. |
| Databáze: | OpenAIRE |
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