Novel solid phase-based ELISA assays contribute to an improved detection of anti-HLA antibodies and to an increased reliability of pre- and post-transplant crossmatching
| Autor: | Oliver Schurat, Beatrix Pollok-Kopp, Till Manzke, Gerald Schlaf, Wolfgang Altermann |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
allograft
Lymphocyte 030232 urology & nephrology complement-dependent cytotoxicity assay Human leukocyte antigen 030230 surgery 03 medical and health sciences 0302 clinical medicine human leukocyte antigen medicine Hla antibodies In-Depth Clinical Review crossmatch Cytotoxicity Pre and post Transplantation medicine.diagnostic_test biology business.industry Virology 3. Good health medicine.anatomical_structure Nephrology Immunology biology.protein rejection Antibody business Tissue typing |
| Zdroj: | NDT Plus |
| ISSN: | 2048-8513 2048-8505 |
| Popis: | Antibodies directed against HLA antigens of a given organ donor represent the dominating reason for hyper-acute or acute allograft rejections. In order to select recipients without donor-specific antibodies, a standard crossmatch (CM) procedure, the complement-dependent cytotoxicity assay (CDC), was developed. This functional assay strongly depends on the availability of isolated vital lymphocytes of a given donor. However, the requirements of the donor’s material may often not be fulfilled, so that the detection of the antibodies directed against HLA molecules is either impaired or becomes completely impossible. To circumvent the disadvantages of the CDC procedure, enzyme-linked immunosorbent assay (ELISA)-based and other solid phase-based ELISA-related techniques have been designed to reliably detect anti-HLA antibodies in recipients. Due to the obvious advantages of these novel technologies, when compared with the classical CDC assay, there is an urgent need to implement them as complementary methods or even as a substitution for the conventional CDC crossmatch that is currently being applied by all tissue typing laboratories. |
| Databáze: | OpenAIRE |
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