Shear stress influences the release of platelet derived growth factor and basic fibroblast growth factor by arterial smooth muscle cells
| Autor: | Antonio V. Sterpetti, Sandro Lepidi, Alessandra Fragale, Alessandra Cucina, Antonino Cavallaro, Luciana Santoro-D'Angelo |
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| Přispěvatelé: | Sterpetti, Av, Cucina, A, Fragale, A, Lepidi, S, Cavallaro, A, Santoro D’Angelo, L |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Time Factors Arteriosclerosis Cells Basic fibroblast growth factor Fibroblast growth factor Awards and Prizes Hemodynamics In Vitro Techniques Stress chemistry.chemical_compound Atherosclerosis Platelet derived growth factor Shear stress Smooth muscle cells Animals Cattle Cells Cultured Europe Fibroblast Growth Factor 2 Muscle Smooth Vascular Platelet-Derived Growth Factor Societies Medical Stress Mechanical Vascular Surgical Procedures Internal medicine Vascular Medical Medicine Cultured biology business.industry Mechanical Fibronectin Endocrinology chemistry biology.protein Biophysics Muscle Smooth Cardiology and Cardiovascular Medicine business Thymidine Societies Platelet-derived growth factor receptor |
| Zdroj: | Scopus-Elsevier |
| Popis: | Objectives: To determine the correlation between haemodynamic forces and the release of two mitogens for smooth muscle cells (SMC): Platelet Derived Growth Factor (PDGF) and basic Fibroblast Growth Factor (bFGF). Methodology: Bovine aortic smooth muscle cells were seeded on fibronectin coated polystyrene cylinders and allowed to reach confluence. The cells were subjected to a laminar flow of 50cd/min (3 dyne/cm 2 ), 100 cc/min (6 dyne/cm 2 ) and 150 cc/min (9 dyne/cm 2 ) in an in vitro system. Control cells were subjected to similar incubation conditions without flow. Principal results: Shear stress increased the release of mitogens by SMC. The release of mitogens was proportional to the level of shear stress and was still evident 24 hours after flow cessation. Conditioned serum-free medium from SMC subjected to shear stress increased tritiated thymidine uptake in Swiss 3T3 fibroblasts 13-fold as compared to conditioned serum-free medium from control SMC not subjected to shear stress ( p p p p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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