Molecular Comparison and Evolutionary Analyses of VP1 Nucleotide Sequences of New African Human Enterovirus 71 Isolates Reveal a Wide Genetic Diversity
| Autor: | Marie-Line Joffret, Jagadish M. Deshpande, Richter Razafindratsimandresy, Maël Bessaud, Francis Delpeyroux, Antoine Nougairède, Xavier de Lamballerie, Jean-Luc Bailly, Audrey Dubot-Pérès, Jean-Michel Heraud |
|---|---|
| Přispěvatelé: | Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP), Biologie des virus entériques (BVE), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Enterovirus Research Centre, Indian Council of Medical Research [New Dehli] (ICMR), Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahidol University [Bangkok]-Mahosot Hospital, Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Unité de Virologie [Antananarivo, Madagascar] (IPM), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Biologie des Virus entériques (BVE), Institut Pasteur [Paris] (IP), Université d'Auvergne - Clermont-Ferrand I (UdA), Financial support for this project was provided by the Agence Interetablissements de Recherche pour le Développement in Madagascar and by the Agence Nationale de la Recherche (ANR-09-MIEN-019) and the Fondation pour la Recherche Médicale (DMI20091117313) in France, ANR-09-MIEN-0019,RecPolioCoxEmerge,Recombinaison entre poliovirus et entérovirus humain de l'espèce C - un nouveau modèle d'évolution et d'émergence(2009), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], University of Oxford [Oxford], Institut Pasteur [Paris], Rakotoarison, Princy, MIE : Maladies infectieuses, immunité et environnement - Recombinaison entre poliovirus et entérovirus humain de l'espèce C - un nouveau modèle d'évolution et d'émergence - - RecPolioCoxEmerge2009 - ANR-09-MIEN-0019 - MIE - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Viral Diseases
MESH: Enterovirus A Human/genetics Time Factors viruses MESH: Africa MESH: Base Sequence Divergent Evolution MESH: Genotype MESH: Madagascar fluids and secretions Emerging Viral Diseases MESH: Genetic Variation MESH: Models Genetic MESH: Enterovirus A Human/isolation & purification 10. No inequality MESH: Phylogeny Phylogeny MESH: Evolution Molecular Genetics [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology 0303 health sciences Multidisciplinary Phylogenetic tree 1. No poverty virus diseases Phylogenetics Infectious Diseases Viral evolution GenBank [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine Research Article Genotype Sequence analysis Science MESH: Bayes Theorem Biology Forms of Evolution Microbiology Viral Evolution Hand Foot and Mouth Disease Evolution Molecular Viral Proteins 03 medical and health sciences stomatognathic system Virology Genetic variation Madagascar Humans Evolutionary Systematics 030304 developmental biology Evolutionary Biology Genetic diversity MESH: Humans Base Sequence Models Genetic 030306 microbiology MESH: Time Factors Genetic Variation Bayes Theorem digestive system diseases Enterovirus A Human Genetic divergence Viral Classification MESH: Viral Proteins/genetics Africa |
| Zdroj: | PLoS ONE PLoS ONE, 2014, 9 (3), pp.e90624. ⟨10.1371/journal.pone.0090624⟩ PLoS ONE, Public Library of Science, 2014, 9 (3), pp.e90624. ⟨10.1371/journal.pone.0090624⟩ PLoS ONE, Vol 9, Iss 3, p e90624 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0090624⟩ |
| Popis: | International audience; Most circulating strains of Human enterovirus 71 (EV-A71) have been classified primarily into three genogroups (A to C) on the basis of genetic divergence between the 1D gene, which encodes the VP1 capsid protein. The aim of the present study was to provide further insights into the diversity of the EV-A71 genogroups following the recent description of highly divergent isolates, in particular those from African countries, including Madagascar. We classified recent EV-A71 isolates by a large comparison of 3,346 VP1 nucleotidic sequences collected from GenBank. Analysis of genetic distances and phylogenetic investigations indicated that some recently-reported isolates did not fall into the genogroups A-C and clustered into three additional genogroups, including one Indian genogroup (genogroup D) and 2 African ones (E and F). Our Bayesian phylogenetic analysis provided consistent data showing that the genogroup D isolates share a recent common ancestor with the members of genogroup E, while the isolates of genogroup F evolved from a recent common ancestor shared with the members of the genogroup B. Our results reveal the wide diversity that exists among EV-A71 isolates and suggest that the number of circulating genogroups is probably underestimated, particularly in developing countries where EV-A71 epidemiology has been poorly studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|