Salvianolic acid B protects hepatocytes from H2O2injury by stabilizing the lysosomal membrane
| Autor: | Yi-Lu Jiang, Xiao-Feng Yan, Jiao-Jiao Luo, Xiao-Ling Wang, Pei Zhao, Liu Liu, Dong-Yan Ma |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
inorganic chemicals 0301 basic medicine Lysosomal membrane Cell Membrane Permeability Cell Survival Blotting Western Cathepsin A Injury Apoptosis Salvia miltiorrhiza Real-Time Polymerase Chain Reaction Cathepsin B Cell Line Protein Carbonylation Mice 03 medical and health sciences Cytosol medicine Animals Humans Hepatocyte Carbon Tetrachloride Benzofurans Lysosomal membrane permeabilization Mice Inbred BALB C Salvianolic acid B Chemistry Gastroenterology Lysosome-Associated Membrane Glycoproteins Alanine Transaminase Hydrogen Peroxide Intracellular Membranes General Medicine Basic Study Flow Cytometry 030104 developmental biology medicine.anatomical_structure Liver Biochemistry Hepatocytes Chemical and Drug Induced Liver Injury Lysosomes Drugs Chinese Herbal Signal Transduction |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v23.i29.5333 |
| Popis: | AIM To investigate the capability of salvianolic acid B (Sal B) to protect hepatocytes from hydrogen peroxide (H2O2)/carbon tetrachloride (CCl4)-induced lysosomal membrane permeabilization. METHODS Cell Counting Kit-8 assay was used to measure cell viability. Apoptosis and death were assayed through flow cytometry. BrdU incorporation was used to detect cell proliferation. Serum alanine aminotransferase activity and liver malondialdehyde (MDA) content were measured. Liver histopathological changes were evaluated using hematoxylin-eosin staining. Lysosomal membrane permeability was detected with LysoTracker Green-labeled probes and acridine orange staining. The levels of protein carbonyl content (PCC), cathepsins (Cat)B/D, and lysosome-associated membrane protein 1 (LAMP1) were evaluated through western blotting. Cytosol CatB activity analysis was performed with chemiluminescence detection. The mRNA level of LAMP1 was evaluated through quantitative real-time polymerase chain reaction. RESULTS Results indicated that H2O2 induced cell injury/death. Sal B attenuated H2O2-induced cell apoptosis and death, restored the inhibition of proliferation, decreased the amount of PCC, and stabilized the lysosome membrane by increasing the LAMP1 protein level and antagonizing CatB/D leakage into the cytosol. CCl4 also triggered hepatocyte death. Furthermore, Sal B effectively rescued hepatocytes by increasing LAMP1 expression and by reducing lysosomal enzyme translocation to the cytosol. CONCLUSION Sal B protected mouse embryonic hepatocytes from H2O2/CCl4-induced injury/death by stabilizing the lysosomal membrane. |
| Databáze: | OpenAIRE |
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