OTIMIZAÇÃO DO PROCESSO NÃO INVASIVO E NÃO DESTRUTIVO DE EXTRAÇÃO E ANÁLISE DE METABÓLITOS VOLÁTEIS DE MAMÃO PAPAIA POR SPME-GC-MS
| Autor: | Daniel Terao, Larissa R. Terra, Sonia C. N. Queiroz, Márcia M. C. Ferreira |
|---|---|
| Přispěvatelé: | LARISSA ROCHA TERRA, IQ-UNICAMP, MARCIA MIGUEL CASTRO FERREIRA, IQ-UNICAMP, DANIEL TERAO, CNPMA, SONIA CLAUDIA DO N DE QUEIROZ, CNPMA. |
| Rok vydání: | 2020 |
| Předmět: |
business.product_category
Central composite design Response surface methodology Metabolomics Solid-phase microextraction response surface methodology Microbial detection chemistry.chemical_compound Cromatografia Gasosa Metabolites Bottle Espectrometria Chemical analysis Volatile organic compounds Fiber QD1-999 Análise Química Carica Papaya Gas chromatography Papayas Chromatography Mass spectrometry Polydimethylsiloxane Mamão Extraction (chemistry) Solid phase microextraction Microrganismo General Chemistry Divinylbenzene metabolomics Chemistry chemistry Fruit business |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Química Nova, Vol 43, Iss 9, Pp 1240-1245 (2020) Química Nova, Volume: 43, Issue: 9, Pages: 1240-1245, Published: 28 OCT 2020 Química Nova v.43 n.9 2020 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.21577/0100-4042.20170611 |
| Popis: | The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 μm polydimethylsiloxane (PDMS) and a 65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB). The PDMS/DVB fiber extracted the highest number of volatiles. Central composite design (CCD) was performed to assess the effect of papaya conditioning time and the fiber exposure time on the response: number of detectable peaks. Both variables studied and their interactions were significant for the response. ANOVA showed that the quadratic terms and the lack of fit were not significant (p |
| Databáze: | OpenAIRE |
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