Assessing the Archaeoparasitological Potential of Quids As a Source Material for Immunodiagnostic Analyses
| Autor: | Johnica J. Morrow, Karl J. Reinhard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saliva Archaeoparasitology Trypanosoma cruzi 030231 tropical medicine Toxoplasma gondii Antibodies Protozoan Enzyme-Linked Immunosorbent Assay quid 03 medical and health sciences 0302 clinical medicine parasitic diseases Source material archaeoparasitology Humans Secretory IgA Mexico La Cueva de los Muertos Chiquitos archaeoserology biology Fossils biology.organism_classification 030104 developmental biology Infectious Diseases Elisa test Immunology biology.protein Special Section on Paleoparasitology Parasitology Original Article ELISA Antibody Toxoplasma |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | In the present study, quids from La Cueva de los Muertos Chiquitos (CMC) were subjected to ELISA tests for 2 protozoan parasites, Toxoplasma gondii (n=45) and Trypanosoma cruzi (n=43). The people who occupied CMC, the Loma San Gabriel, lived throughout much of present-day Durango and Zacatecas in Mexico. The known pathoecology of these people puts them into at-risk categories for the transmission of T. gondii and T. cruzi. Human antibodies created in response to these 2 parasites can be detected in modern saliva using ELISA kits intended for use with human serum. For these reasons, quids were reconstituted and subjected to ELISA testing. All test wells yielded negative results. These results could be a factor of improper methods because there is no precedence for this work in the existing literature. The results could equally be a simple matter of parasite absence among those people who occupied CMC. A final consideration is the taphonomy of human antibodies and whether or not ELISA is a sufficient method for recovering antibodies from archaeological contexts. An additional ELISA test targeting secretory IgA (sIgA) was conducted to further examine the failure to detect parasite-induced antibodies from quids. Herein, the methods used for quid preparation and ELISA procedures are described so that they can be further developed by future researchers. The results are discussed in light of the potential future of quid analysis. |
| Databáze: | OpenAIRE |
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