Expansion of peripheral blood natural killer cells correlates with clinical outcome in cancer patients receiving recombinant subcutaneous interleukin-2 and interferon-alpha-2
| Autor: | Jens Atzpodien, Jens Grosse, Matthias Volkenandt, Martin Hadam, Alfred Körfer, Anke Franzke, Hubert Poliwoda, Markus Deckert, Iris Dallmann, Axel Schomburg, Hartmut Kirchner, Thomas Menzel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Interleukin 2
Lymphoma B-Cell medicine.medical_treatment Lymphocyte Injections Subcutaneous Alpha interferon Interferon alpha-2 Drug Administration Schedule Natural killer cell Immunophenotyping Cancer immunotherapy Antigens CD T-Lymphocyte Subsets Neoplasms medicine Humans Carcinoma Renal Cell Melanoma Interferon alfa business.industry Interferon-alpha General Medicine Immunotherapy Hodgkin Disease Kidney Neoplasms Recombinant Proteins Killer Cells Natural medicine.anatomical_structure Treatment Outcome Immunology Interleukin-2 business Colorectal Neoplasms medicine.drug |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 14(6) |
| ISSN: | 1010-4283 |
| Popis: | Natural killer (NK) cells are believed to contribute to the clinical efficacy of cancer immunotherapy using recombinant interleukin-2 (rIL-2) in humans. In previous trials of high-dose i.v. rIL-2, however, no correlation has been established between circulating NK cells and treatment response. Between January 1989 and October 1990, we treated a total of 47 outpatients with advanced tumors using low-dose s.c. rIL-2 and interferon-alpha-2 (rIFN-alpha). Therapy consisted of a 2-day rIL-2 pulse at 18 million IU/m2/day, followed by 6 weeks of rIL-2 (3.6 x 10(6)-4.8 x 10(6) IU/m2/day x 5 days/week) and rIFN-alpha (5 x 10(6)-6 x 10(6) U/m2 x 3/week). Before and after therapy, we phenotypically evaluated circulating lymphocytes and correlated them with clinical response. During 6-week therapy, peripheral blood lymphocytes bearing the CD56 (NK-cell-associated) surface antigen were increased significantly (por = 0.005) in treatment responders [complete response (CR) and partial response (PR), n = 10; 3.8-fold] and stable disease (SD) patients (n = 20; 2.1-fold), while patients with progressive disease (PD, n = 17) exhibited no significant expansion of circulating NK cells (p0.1). After one 6-week treatment cycle, CR/PR patients had significantly more peripheral NK cells, when compared with patients in SD (1.6-fold) and PD (1.9-fold) (p0.04). The overall number of circulating lymphocytes was also increased upon therapy (1.6-fold; por = 0.001), but remained independent of response (p0.4). These data demonstrate that s.c. rIL-2 and s.c. rIFN-alpha produce a significant increase in peripheral blood NK cells; this expansion correlates significantly with treatment response in advanced tumor patients receiving long-term combination immunotherapy at outpatient doses. |
| Databáze: | OpenAIRE |
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