miR-9-5p Exerts a Dual Role in Cervical Cancer and Targets Transcription Factor TWIST1
| Autor: | Renske D.M. Steenbergen, Annina P van Splunter, Annelieke Jaspers, Iris A E van der Hoorn, Saskia M Wilting, Iris Babion |
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| Přispěvatelé: | Medical Oncology, Pathology, CCA - Cancer biology and immunology, AII - Cancer immunology, CCA - Imaging and biomarkers |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
HPV cervical cancer Uterine Cervical Neoplasms TWIST1 Biology Article HeLa 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Cell Line Tumor microRNA medicine Humans Gene silencing lcsh:QH301-705.5 3' Untranslated Regions Transcription factor Cell Proliferation Cervical cancer Human papillomavirus 16 Human papillomavirus 18 Twist-Related Protein 1 Nuclear Proteins General Medicine Methylation DNA Methylation medicine.disease biology.organism_classification miR-9-5p 3. Good health Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology lcsh:Biology (General) Cell culture 030220 oncology & carcinogenesis Cancer research Adenocarcinoma Female RNA Interference methylation |
| Zdroj: | Cells, 9(1):65. Multidisciplinary Digital Publishing Institute (MDPI) Cells, 9(1):65. MDPI Multidisciplinary Digital Publishing Institute Cells Cells, Vol 9, Iss 1, p 65 (2019) Volume 9 Issue 1 Babion, I, Jaspers, A, van Splunter, A P, van der Hoorn, I A E, Wilting, S M & Steenbergen, R D M 2020, ' MiR-9-5p exerts a dual role in cervical cancer and targets transcription factor TWIST1 ', Cells, vol. 9, no. 1, 65 . https://doi.org/10.3390/cells9010065 |
| ISSN: | 2073-4409 |
| Popis: | Squamous cell carcinoma (SCC) and adenocarcinoma (AC) represent the major cervical cancer histotypes. Both histotypes are caused by infection with high-risk HPV (hrHPV) and are associated with deregulated microRNA expression. Histotype-dependent expression has been observed for miR-9-5p, showing increased expression in SCC and low expression in AC. Here, we studied the regulation and functionality of miR-9-5p in cervical SCCs and ACs using cervical tissue samples and hrHPV-containing cell lines. Expression and methylation analysis of cervical tissues revealed that low levels of miR-9-5p in ACs are linked to methylation of its precursor genes, particularly miR-9-1. Stratification of tissue samples and hrHPV-containing cell lines suggested that miR-9-5p depends on both histotype and hrHPV type, with higher expression in SCCs and HPV16-positive cells. MiR-9-5p promoted cell viability and anchorage independence in cervical cancer cell lines SiHa (SCC, HPV16) and CaSki (metastasized SCC, HPV16), while it played a tumor suppressive role in HeLa (AC, HPV18). TWIST1, a transcription factor involved in epithelial-to-mesenchymal transition (EMT), was established as a novel miR-9-5p target. Our results show that miR-9-5p plays a dual role in cervical cancer in a histotype- and hrHPV type-dependent manner. MiR-9-5p mediated silencing of TWIST1 suggests two distinct mechanisms towards EMT in cervical cancer. |
| Databáze: | OpenAIRE |
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