Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation
| Autor: | Robert A. Floyd, Peter K. Wong, Julia J. Watson, Dale H. Altmiller, Robert C. Rickard |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
biology
Ethanol Free Radicals Chemistry Hydroxyl Radical Radical Thiourea 8-Hydroxy-2'-deoxyguanosine Deoxyguanosine Free Radical Scavengers Photochemistry Biochemistry Ferrous Adduct Superoxide dismutase chemistry.chemical_compound Radical ion 8-Hydroxy-2'-Deoxyguanosine biology.protein Electrochemistry Chromatography High Pressure Liquid |
| Zdroj: | Free radical research communications. 1(3) |
| ISSN: | 8755-0199 |
| Popis: | DNA or 2-deoxyguanosine reacts with hydroxyl free radical to form 8-hydroxy-deoxyguanosine (8-OH-dG). We found that 8-OH-dG can be effectively separated from deoxyguanosine by high pressure liquid chromatography and very sensitively detected using electrochemical detection. The sensitivity of electrochemical detection is about one-thousand fold enhanced over optical detection. Utilizing deoxyguanosine in bicarbonate buffer it was found that ferrous ion, but not ferric ion, was effective in forming 8-OH-dG. The hydroxyl free radical scavenging agents, thiourea and ethanol, were very effective in quenching Fe(11) mediated 8-OH-dG formation, but superoxide dismutase had very little effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|