Differential MHC Class II Presentation of a Pathogenic Autoantigen during Health and Disease
| Autor: | Fei F. Shih, Jennifer L. Racz, Paul M. Allen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
T-Lymphocytes
Immunology Antigen-Presenting Cells Arthritis Mice Transgenic Cell Separation Autoantigens Mice Immune system In vivo Animals Immunology and Allergy Medicine Antigen-presenting cell Cells Cultured Inflammation Antigen Presentation B-Lymphocytes MHC class II biology business.industry Glucose-6-Phosphate Isomerase Histocompatibility Antigens Class II medicine.disease Immune complex Mice Inbred C57BL Polyclonal B cell response Health Polyclonal antibodies Acute Disease biology.protein business |
| Zdroj: | The Journal of Immunology. 176:3438-3448 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.176.6.3438 |
| Popis: | Glucose-6-phosphate isomerase (GPI) is the target autoantigen recognized by KRN T cells in the K/BxN model of rheumatoid arthritis. T cell reactivity to this ubiquitous Ag results in the recruitment of anti-GPI B cells and subsequent immune complex-mediated arthritis. Because all APCs have the capacity to process and present this autoantigen, it is unclear why systemic autoimmunity with polyclonal B cell activation does not ensue. To this end, we examined how GPI is presented by B cells relative to other immunologically relevant APCs such as dendritic cells (DCs) and macrophages in the steady state, during different phases of arthritis development, and after TLR stimulation. Although all APCs can process and present the GPI:I-Ag7 complex, they do so with different efficiencies. DCs are the most potent at baseline and become progressively more potent with disease development correlating with immune complex uptake. Interestingly, in vivo and in vitro maturation of DCs did not enhance GPI presentation, suggesting that DCs use mechanisms to regulate the presentation of self-peptides. Non-GPI-specific B cells are the weakest APCs (100-fold less potent than DCs) and fail to productively engage KRN T cells at steady state and during arthritis. However, the ability to stimulate KRN T cells is strongly enhanced in B cells after TLR ligation and provides a mechanism whereby polyclonal B cells may be activated in the wake of an acute infection. |
| Databáze: | OpenAIRE |
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