Naturally Occurring Plasmids in Acinetobacter calcoaceticus: pAV2, a Plasmid which Influences the Fertility of the Sex Factor pAV1
| Autor: | Marilyn E. Nugent, Edward Hinchliffe, Alan Vivian |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Genetics
Acinetobacter biology Molecular mass Strain (chemistry) Drug Resistance Microbial biology.organism_classification Microbiology Molecular biology Molecular Weight F Factor chemistry.chemical_compound Plasmid Cryptic plasmid chemistry Conjugation Genetic Agarose gel electrophoresis Agarose Acinetobacter calcoaceticus Host restriction Plasmids |
| Zdroj: | Microbiology. 121:411-418 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-121-2-411 |
| Popis: | Acinetobacter calcoaceticus strain EBF65/65 harbours a cryptic plasmid, pAV2, which has been shown by electrophoretic separation on agarose gels to have a molecular mass of approximately 13.5 megadaltons (Md). Transfer of the previously described sex factor pAV1 (Hinchliffe & Vivian, 1980a, b) from the hospital strain JC17 into strains possessing pAV2 occurs only at a low frequency, whereas transfer to similar strains lacking pAV2 occurs at a much higher frequency. In EBF65/65, pAV1 may be present in strains possessing or lacking pAV2; pAV1 strains lacking pAV2 correspond to strains previously described as pAV1a (Hinchliffe & Vivian, 1980b) whereas pAV1 strains which also possess pAV2 correspond to pAV1b strains. The genetic evidence presented here is consistent with the hypothesis that pAV2 specifies a host restriction and modification system that is active against pAV1. Physical evidence from agarose gel electrophoresis indicates that pAV1 corresponds to a band of approximately 85 Md in strain JC17. The corresponding band in strains of EBF65/65 is difficult to distinguish because of the presence of a further cryptic plasmid band of approximately 88 Md, designated pAV3. A small cryptic plasmid of approximately 6 Md, designated pAV4, is reported for EBF65/65. |
| Databáze: | OpenAIRE |
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