Transgenic Expression of dsRNA Targeting the Pentalonia nigronervosa acetylcholinesterase Gene in Banana and Plantain Reduces Aphid Populations
| Autor: | Jaindra Nath Tripathi, Benjamin Dugdale, Leena Tripathi, Edward K. Muge, George Obiero, Temitope Jekayinoluwa, James L. Dale |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Transgene Plant Science 01 natural sciences Article 03 medical and health sciences RNA interference Gene Ecology Evolution Behavior and Systematics banana aphid 030304 developmental biology 0303 health sciences Pentalonia nigronervosa Aphid Ecology biology plantain fungi Botany food and beverages acetylcholinesterase biochemical phenomena metabolism and nutrition biology.organism_classification artificial diet Virology Banana bunchy top virus RNA silencing banana sugars QK1-989 PEST analysis 010606 plant biology & botany |
| Zdroj: | Plants Plants, Vol 10, Iss 613, p 613 (2021) Volume 10 Issue 4 |
| ISSN: | 2223-7747 |
| Popis: | The banana aphid,Pentalonia nigronervosa, is the sole insect vector of banana bunchy top virus (BBTV), the causal agent of banana bunchy top disease. The aphid acquires and transmits BBTV while feeding on infected banana plants. RNA interference (RNAi) enables the generation of pest and disease-resistant crops however, its effectiveness relies on the identification of pivotal gene sequences to target and silence. Acetylcholinesterase (AChE) is an essential enzyme responsible for the hydrolytic metabolism of the neurotransmitter acetylcholine in animals. In this study, the AChE gene of the banana aphid was targeted for silencing by RNAi through transgenic expression of AChE dsRNA in banana and plantain plants. The efficacy of dsRNA was first assessed using an artificial feeding assay. In vitro aphid feeding on a diet containing 7.5% sucrose, and sulfate complexes of trace metals supported aphid growth and reproduction. When AChE dsRNA was included in the diet, a dose of 500 ng/μL was lethal to the aphids. Transgenic banana cv. Cavendish Williams and plantain cvs. Gonja Manjaya and Orishele expressing AChE dsRNA were regenerated and assessed for transgene integration and copy number. When aphids were maintained on elite transgenic events, there was a 67.8%, 46.7%, and 75.6% reduction in aphid populations growing on Cavendish Williams, Gonja Manjaya, and Orishele cultivars, respectively, compared to those raised on nontransgenic control plants. These results suggest that RNAi targeting an essential aphid gene could be a useful means of reducing both aphid infestation and potentially the spread of the disease they transmit. |
| Databáze: | OpenAIRE |
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