Astragalus Polysaccharide Protects Against Cadmium-Induced Autophagy Injury Through Reactive Oxygen Species (ROS) Pathway in Chicken Embryo Fibroblast
| Autor: | Zequn Tang, Ruili Zhang, Xudong Shen, Meishuo Wan, Yu Bai, Ming Ge, Miao Yu, Guangxing Li, Jingyi Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
animal structures
Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Chick Embryo 010501 environmental sciences Cadmium chloride medicine.disease_cause 01 natural sciences Biochemistry Cadmium poisoning Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Polysaccharides Autophagy medicine Animals Cell damage PI3K/AKT/mTOR pathway 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences Reactive oxygen species 030302 biochemistry & molecular biology Biochemistry (medical) General Medicine Fibroblasts medicine.disease Cell biology chemistry embryonic structures Reactive Oxygen Species Chickens Oxidative stress Cadmium |
| Zdroj: | Biological Trace Element Research. 200:318-329 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/s12011-021-02628-y |
| Popis: | Cadmium (Cd) is a harmful heavy metal pollutant, which can cause oxidative stress in the body and induce cell damage. Reactive oxygen species (ROS) is a general term for substances that contain oxygen and are active in the body. However, excessive ROS can damage the body. Cadmium poisoning can cause a large amount of ROS in cells and autophagy. Astragalus polysaccharide (APS) is a plant polysaccharide with biological functions, such as antioxidant and anti-stress activities. In this study, chicken embryo fibroblasts (CEF) were used to determine the relationship between ROS and autophagy damage of Cd-infected cells and the mechanism of APS on cadmium-induced autophagy damage. The results showed that a 10-μL dose of 10 μmol/L cadmium chloride (CdCl2) can induce CEF autophagy and damage when CEF was added for 36 h. Cadmium induced CEF autophagy damage by increasing ROS production. APS could significantly reduce ROS production and LC3-II and Beclin-1 protein expression, increase the expression of mTOR and the level of antioxidation, and restore the viability and morphological damage of CEF exposed to Cd. Our study suggests that APS can alleviate Cd-induced CEF autophagy damage by reducing the production of ROS. |
| Databáze: | OpenAIRE |
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