Targeting the Mild-Hypoxia Driving Force for Metabolic and Muscle Transcriptional Reprogramming of Gilthead Sea Bream (Sparus aurata) Juveniles
| Autor: | Josep A. Calduch-Giner, Verónica de las Heras, Juan Antonio Martos-Sitcha, Fernando Naya-Català, Jaume Pérez-Sánchez, Paula Simó-Mirabet |
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| Přispěvatelé: | European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Biología |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Metabolic landmarks Protein turnover Cellular respiration QH301-705.5 growth Muscle transcriptome Physiology Growth Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Swimming performance 0302 clinical medicine lipid metabolism medicine gilthead sea bream Glycolysis 14. Life underwater hypo-metabolic state Biology (General) Hypoxia Phenotypic plasticity General Immunology and Microbiology hypoxia protein turnover Hypoxia (medical) glycolysis muscle transcriptome 030104 developmental biology Lipid metabolism metabolic landmarks Gilthead sea bream swimming performance Respirometer medicine.symptom General Agricultural and Biological Sciences Anaerobic exercise 030217 neurology & neurosurgery Hypo-metabolic state Muscle contraction |
| Zdroj: | Biology, Vol 10, Iss 416, p 416 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname Biology 2021, 10(5), 416 Biology Volume 10 Issue 5 RODIN. Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz RODIN: Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz Universidad de Cádiz |
| ISSN: | 2079-7737 |
| Popis: | © 2021 by the authors. On-growing juveniles of gilthead sea bream were acclimated for 45 days to mild-hypoxia (M-HYP, 40–60% O2 saturation), whereas normoxic fish (85–90% O2 saturation) constituted two different groups, depending on if they were fed to visual satiety (control fish) or pair-fed to M-HYP fish. Following the hypoxia conditioning period, all fish were maintained in normoxia and continued to be fed until visual satiation for 3 weeks. The time course of hypoxia-induced changes was assessed by changes in blood metabolic landmarks and muscle transcriptomics before and after exhaustive exercise in a swim tunnel respirometer. In M-HYP fish, our results highlighted a higher contribution of aerobic metabolism to whole energy supply, shifting towards a higher anaerobic fitness following normoxia restoration. Despite these changes in substrate preference, M-HYP fish shared a persistent improvement in swimming performance with a higher critical speed at exercise exhaustion. The machinery of muscle contraction and protein synthesis and breakdown was also largely altered by mild-hypoxia conditioning, contributing this metabolic re-adjustment to the positive regulation of locomotion and to the catch-up growth response during the normoxia recovery period. Altogether, these results reinforce the presence of large phenotypic plasticity in gilthead sea bream, and highlights mild-hypoxia as a promising prophylactic measure to prepare these fish for predictable stressful events. This work was financially supported by a grant from the European Commission of the European Union under the Horizon 2020 research infrastructure project AQUAEXCEL2020 (652831) to J.P-S. Additional funding was obtained by a Spanish MICINN project (Bream-AquaINTECH, RTI2018–094128-B-I00). J.A.M.-S. received a Postdoctoral Research Fellowship (Juan de la CiervaFormación, Reference FJCI-2014-20,161). |
| Databáze: | OpenAIRE |
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