Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus
| Autor: | David Hernandez, Patrick Linder, Antoine Huyghe, James E. Cassat, Adrien Nicolas Fischer, Ludwig Stenz, Jacques Schrenzel, Manuela Tangomo, Patrice Francois |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus aureus
Micrococcaceae Population Sigma Factor medicine.disease_cause Staphylococcal infections Microbiology Bacterial Adhesion 03 medical and health sciences chemistry.chemical_compound Biofilms/drug effects Oleic Acid/pharmacology Bacterial Proteins Genetics medicine Humans education Molecular Biology Sigma Factor/genetics/metabolism 030304 developmental biology Staphylococcus carnosus ddc:616 0303 health sciences education.field_of_study Microbial Viability biology 030306 microbiology Bacterial Adhesion/drug effects Microbial Viability/drug effects Biofilm Staphylococcal Infections biology.organism_classification medicine.disease Staphylococcal Infections/microbiology Oleic acid chemistry Biofilms Bacterial Proteins/genetics/metabolism Staphylococcus aureus/drug effects/physiology Bacteria Oleic Acid |
| Zdroj: | FEMS Microbiology Letters, Vol. 287, No 2 (2008) pp. 149-55 |
| ISSN: | 0378-1097 |
| Popis: | Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. aureus clinical isolates show the capacity to adhere to abiotic surfaces and to develop biofilms. Because S. aureus growing in a biofilm is highly refractory to treatment, inhibition of biofilm formation represents a major therapeutic objective. We evaluated the effects of oleic acid on primary adhesion and biofilm production in eight genotypically different S. aureus strains as well as in the biofilm-negative Staphylococcus carnosus strain TM300. Oleic acid inhibited primary adhesion but increased biofilm production in every S. aureus strain tested. Staphylococcus aureus strain UAMS-1 was then selected as a model organism for studying the mechanisms triggered by oleic acid on the formation of a biofilm in vitro. Oleic acid inhibited the primary adhesion of UAMS-1 dose dependently with an IC(50) around 0.016%. The adherent bacterial population decreased proportionally with increasing concentrations of oleic acid whereas an opposite effect was observed on the planktonic population. Overall, the total bacterial counts remained stable. Macroscopic detachments and clumps were visible from the adherent bacterial population. In the presence of oleic acid, the expression of sigB, a gene potentially involved in bacterial survival through an effect on fatty acid composition, was not induced. Our results suggest a natural protective effect of oleic acid against primary adhesion. |
| Databáze: | OpenAIRE |
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