A detailed comparison between the endoscopic images using blue laser imaging and three-dimensional reconstructed pathological images of colonic lesions

Autor: Kohei Morita, Chiho Ohbayashi, Yuichi Teramura, Yayoi Matsumoto, Yoshiyuki Sasaki, Maiko Takeda, Hisao Fujii, Tadashi Nakagawa, Takashi Inoue, Shinji Nakamura, Takeshi Ueda, Fumikazu Koyama, Takayuki Nakamoto, Masayuki Sho, Hiroyuki Kuge
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Endoscope
01 natural sciences
Narrow Band Imaging
0302 clinical medicine
Adenocarcinomas
Medicine and Health Sciences
Early Detection of Cancer
Multidisciplinary
medicine.diagnostic_test
Middle Aged
Adenomas
Oncology
Colonic Neoplasms
Medicine
Female
030211 gastroenterology & hepatology
Anatomy
Research Article
Endoscopic image
Histology
Colon
Imaging Techniques
Science
Surgical and Invasive Medical Procedures
Adenocarcinoma
Research and Analysis Methods
Carcinomas
010309 optics
03 medical and health sciences
Imaging
Three-Dimensional
0103 physical sciences
Visible blood
medicine
Humans
Surface structure
Colonic adenocarcinoma
Pathological
Aged
Surgical Resection
business.industry
Biology and Life Sciences
Cancers and Neoplasms
Endoscopy
Gastrointestinal Tract
Cardiovascular Anatomy
Colon tissue
Blood Vessels
Nuclear medicine
business
Digestive System
Zdroj: PLoS ONE, Vol 15, Iss 6, p e0235279 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Blue laser/light imaging (BLI) is an image-enhanced endoscopy (IEE) technique that can provide an accurate diagnosis by closely observing the surface structure of various colonic lesions. However, complete correspondence between endoscopic images and pathological images has not been demonstrated. The aim of this study was to accurately compare endoscopic images and the pathological images using a three-dimensionally (3D) reconstructed pathological model. Continuous thin layer sections were prepared from colonic tissue specimens and immunohistochemically stained for CD34 and CAM5.2. Three-dimensional reconstructed images were created by superimposing immunohistochemically stained pathological images. The endoscopic image with magnifying BLI was compared with the top view of the 3D reconstructed image to identify any one-to-one correspondence between the endoscopic images and histopathological images using the gland orifices and microvessels as a guide. Using 3D reconstructed pathological images, we were able to identify the location on the endoscope image in cases of colonic adenocarcinoma, adenoma and normal mucosa. As a result, the horizontal plane of the endoscopic image and the vertical plane of the 2D pathological specimen were able to be compared, and we successfully determined the visible blood vessel depth and performed a detailed evaluation on magnifying BLI. Examples are as follows: (1) The median vasculature depth from the mucosal surface that could be recognized as vasculature on magnifying BLI was 29.4 μm. The median depth of unrecognizable vessels on magnifying BLI was 218.8 μm, which was significantly deeper than recognizable vessels. (2) Some brownish structures were suggested to potentially be not only dense vessels, vessel expansions, corrupted vessels but also bleeding or extravasation of erythrocytes. Overall, we demonstrated a new approach to matching endoscopic images and pathological findings using a 3D-reconstructed pathological model immunohistochemically stained for CD34 and CAM5.2. This approach may increase the overall understanding of endoscopic images and positively contribute to making more accurate endoscopic diagnoses.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje