A detailed comparison between the endoscopic images using blue laser imaging and three-dimensional reconstructed pathological images of colonic lesions
Autor: | Kohei Morita, Chiho Ohbayashi, Yuichi Teramura, Yayoi Matsumoto, Yoshiyuki Sasaki, Maiko Takeda, Hisao Fujii, Tadashi Nakagawa, Takashi Inoue, Shinji Nakamura, Takeshi Ueda, Fumikazu Koyama, Takayuki Nakamoto, Masayuki Sho, Hiroyuki Kuge |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Male
Endoscope 01 natural sciences Narrow Band Imaging 0302 clinical medicine Adenocarcinomas Medicine and Health Sciences Early Detection of Cancer Multidisciplinary medicine.diagnostic_test Middle Aged Adenomas Oncology Colonic Neoplasms Medicine Female 030211 gastroenterology & hepatology Anatomy Research Article Endoscopic image Histology Colon Imaging Techniques Science Surgical and Invasive Medical Procedures Adenocarcinoma Research and Analysis Methods Carcinomas 010309 optics 03 medical and health sciences Imaging Three-Dimensional 0103 physical sciences Visible blood medicine Humans Surface structure Colonic adenocarcinoma Pathological Aged Surgical Resection business.industry Biology and Life Sciences Cancers and Neoplasms Endoscopy Gastrointestinal Tract Cardiovascular Anatomy Colon tissue Blood Vessels Nuclear medicine business Digestive System |
Zdroj: | PLoS ONE, Vol 15, Iss 6, p e0235279 (2020) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Blue laser/light imaging (BLI) is an image-enhanced endoscopy (IEE) technique that can provide an accurate diagnosis by closely observing the surface structure of various colonic lesions. However, complete correspondence between endoscopic images and pathological images has not been demonstrated. The aim of this study was to accurately compare endoscopic images and the pathological images using a three-dimensionally (3D) reconstructed pathological model. Continuous thin layer sections were prepared from colonic tissue specimens and immunohistochemically stained for CD34 and CAM5.2. Three-dimensional reconstructed images were created by superimposing immunohistochemically stained pathological images. The endoscopic image with magnifying BLI was compared with the top view of the 3D reconstructed image to identify any one-to-one correspondence between the endoscopic images and histopathological images using the gland orifices and microvessels as a guide. Using 3D reconstructed pathological images, we were able to identify the location on the endoscope image in cases of colonic adenocarcinoma, adenoma and normal mucosa. As a result, the horizontal plane of the endoscopic image and the vertical plane of the 2D pathological specimen were able to be compared, and we successfully determined the visible blood vessel depth and performed a detailed evaluation on magnifying BLI. Examples are as follows: (1) The median vasculature depth from the mucosal surface that could be recognized as vasculature on magnifying BLI was 29.4 μm. The median depth of unrecognizable vessels on magnifying BLI was 218.8 μm, which was significantly deeper than recognizable vessels. (2) Some brownish structures were suggested to potentially be not only dense vessels, vessel expansions, corrupted vessels but also bleeding or extravasation of erythrocytes. Overall, we demonstrated a new approach to matching endoscopic images and pathological findings using a 3D-reconstructed pathological model immunohistochemically stained for CD34 and CAM5.2. This approach may increase the overall understanding of endoscopic images and positively contribute to making more accurate endoscopic diagnoses. |
Databáze: | OpenAIRE |
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