Mycobacterium tuberculosis TetR family transcriptional regulator Rv1019 is a negative regulator of the mfd-mazG operon encoding DNA repair proteins

Autor: Akhil Raj Pushparajan, Ranjit Ramachandran, Ramakrishnan Ajay Kumar, Jijimole Gopi Reji
Rok vydání: 2020
Předmět:
DNA
Bacterial
Models
Molecular
Protein Conformation
alpha-Helical
DNA Repair
Transcription
Genetic
Operon
DNA repair
DNA damage
Genetic Vectors
Mycobacterium smegmatis
Biophysics
Regulator
Gene Expression
Biology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Structural Biology
Genetics
Escherichia coli
Protein–DNA interaction
TetR
Protein Interaction Domains and Motifs
Cloning
Molecular
Promoter Regions
Genetic
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
Binding Sites
030302 biochemistry & molecular biology
Tetracycline Resistance
Cell Biology
Gene Expression Regulation
Bacterial
Hydrogen Peroxide
Mycobacterium tuberculosis
Tetracycline
biology.organism_classification
Recombinant Proteins
Cell biology
Anti-Bacterial Agents
chemistry
Protein Multimerization
DNA Damage
Protein Binding
Zdroj: FEBS lettersReferences. 594(17)
ISSN: 1873-3468
Popis: Rv1019, a member of an uncharacterized tetracycline resistance regulator family of transcriptional regulators of Mycobacterium tuberculosis H37Rv, was found to be differentially expressed during dormancy and reactivation in vitro. In this study, we show that this protein binds to its own promoter and acts as a negative regulator of its own expression. It forms dimers in vitro which is essential for the DNA binding activity. We also show that Rv1019 and downstream genes Rv1020 (mfd) and Rv1021 (mazG) are cotranscribed. Constitutive expression of Rv1019 in M. smegmatis downregulated MSMEG_5423 (mfd) and MSMEG_5422 (mazG), suggesting that Rv1019 negatively regulates these downstream genes which encode key proteins involved in DNA repair. M. smegmatis expressing Rv1019 was found to be sensitive to DNA-damaging environments, suggesting its role in regulating the DNA damage response in mycobacterium.
Databáze: OpenAIRE
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