A novel experimental rat model of peripheral nerve scarring that reliably mimics post-surgical complications and recurring adhesions

Autor: Heinz Redl, Monika Bradl, James Ferguson, Angela Lemke, Dominika Lidinsky, Susanne Wolbank, Carina Penzenstadler, Rudolf Hopf, Thomas Hausner
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Pathology
T-Lymphocytes
Medicine (miscellaneous)
Action Potentials
lcsh:Medicine
Tissue Adhesions
Rats
Sprague-Dawley
0302 clinical medicine
Postoperative Complications
Immunology and Microbiology (miscellaneous)
Fibrosis
Recurrence
Gait
Neurolysis
Nerve fibrosis
Nerve scarring
Chronic pain
Anatomy
Peripheral nerve adhesions
Sciatic Nerve
Peripheral
030220 oncology & carcinogenesis
Female
Sciatic nerve
medicine.symptom
Research Article
lcsh:RB1-214
medicine.medical_specialty
Neuroscience (miscellaneous)
Inflammation
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Cicatrix
medicine
lcsh:Pathology
Animals
Pathological
business.industry
Macrophages
lcsh:R
Nerve injury
Nerve inflammation
medicine.disease
Perineural adhesions
Disease Models
Animal
Glutaral
Nerve Degeneration
business
030217 neurology & neurosurgery
Zdroj: Disease Models & Mechanisms, Vol 10, Iss 8, Pp 1015-1025 (2017)
Disease Models & Mechanisms
ISSN: 1754-8411
1754-8403
Popis: Inflammation, fibrosis and perineural adhesions with the surrounding tissue are common pathological processes following nerve injury and surgical interventions on peripheral nerves in human patients. These features can reoccur following external neurolysis, currently the most common surgical treatment for peripheral nerve scarring, thus leading to renewed nerve function impairment and chronic pain. To enable a successful evaluation of new therapeutic approaches, it is crucial to use a reproducible animal model that mimics the main clinical symptoms occurring in human patients. However, a clinically relevant model combining both histological and functional alterations has not been published to date. We therefore developed a reliable rat model that exhibits the essential pathological processes of peripheral nerve scarring. In our study, we present a novel method for the induction of nerve scarring by applying glutaraldehyde-containing glue that is known to cause nerve injury in humans. After a 3-week contact period with the sciatic nerve in female Sprague Dawley rats, we could demonstrate severe intra- and perineural scarring that resulted in grade 3 adhesions and major impairments in the electrophysiological peak amplitude compared with sham control (P=0.0478). Immunohistochemical analysis of the nerve structure revealed vigorous nerve inflammation and recruitment of T cells and macrophages. Also, distinct nerve degeneration was determined by immunostaining. These pathological alterations were further reflected in significant functional deficiencies, as determined by the analysis of relevant gait parameters as well as the quantification of the sciatic functional index starting at week 1 post-operation (P
Summary: An easily reproducible and reliable rat model for peripheral nerve scarring that allows for the effective testing of new therapeutic strategies.
Databáze: OpenAIRE
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