Two-component systems regulate swarming inPseudomonas aeruginosaPA14
| Autor: | Shubham Joge, Divakar Badal, Singh, Manoj M. Varma, Harshitha S. Kotian, Kollaran Am, Deep Prakash, A. K. Mishra |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
030306 microbiology Pseudomonas aeruginosa musculoskeletal neural and ocular physiology Mutant Swarming (honey bee) Biofilm food and beverages Swarm behaviour Motility biochemical phenomena metabolism and nutrition Biology equipment and supplies medicine.disease_cause Cell biology 03 medical and health sciences Soft agar medicine bacteria 030304 developmental biology |
| DOI: | 10.1101/445015 |
| Popis: | Swarming inPseudomonas aeruginosais a quorum-dependant motility over semi-solid surfaces. On soft agar,P. aeruginosaexhibits a dendritic swarm pattern, with multiple levels of branching. Swarm patterns vary considerably depending upon the experimental design. In the present study, we show that the swarm pattern is plastic and media dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity observed across media. Further, through a targeted screen of 113 genes encoding two-component system (TCS) components, we show that 44 TCS genes regulate PA14 swarming in a contextual fashion. However, only four TCS genes are essential for swarming. Many swarming-defective TCS mutants are highly efficient in biofilm formation indicating an antagonistic relationship between swarming and biofilm states inP. aeruginosa. |
| Databáze: | OpenAIRE |
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