Structural characterization and anti-aging activity of a novel extracellular polysaccharide from fungus Phellinus sp. in a mammalian system
| Autor: | Eric Charles Peterson, Dan yang Li, Xiao-kui Ma, Ying Dun, Dan dan Guo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aging Antioxidant Phellinus Magnetic Resonance Spectroscopy Enzimas medicine.medical_treatment Production engineering Trolox equivalent antioxidant capacity Antioxidantes Apoptosis Polysaccharide medicine.disease_cause Superoxide dismutase 03 medical and health sciences Mice Polysaccharides Spectroscopy Fourier Transform Infrared medicine Animals chemistry.chemical_classification Inflammation Depressive Disorder Major biology Alimentos - Análisis Superoxide Dismutase Basidiomycota Protein Tyrosine Phosphatase Non-Receptor Type 6 Galactose General Medicine biology.organism_classification Catalase Ingeniería de producción Disease Models Animal Oxidative Stress 030104 developmental biology Biochemistry chemistry Nutrición Análisis bioquímico biology.protein Hepatocytes Female Cell aging Oxidative stress Food Science |
| Zdroj: | Repositorio ICESI Universidad ICESI instacron:Universidad ICESI |
| ISSN: | 2042-650X |
| Popis: | Little is known about the chemical structure of purified extracellular polysaccharides from Phellinus sp., a fungal species with known medicinal properties. A combination of IR spectroscopy, methylation analysis and NMR were performed for the structural analysis of a purified extracellular polysaccharide derived from Phellinus sp. culture, denoted as SHP-1, along with an evaluation of the anti-aging effect in vivo of the polysaccharide supplementation. The structure of SHP-1 was established, with a backbone composed of →2,4)-α-d-glucopyranose-(1→ and →2)-β-d-mannopyranose-(1→ and two terminal glucopyranose branches. Biochemical analysis from mammalian animal experiments demonstrated that SHP-1 possesses the ability to enhance antioxidant enzyme activities, such as catalase (CAT) and superoxide dismutase (SOD) activities, Trolox equivalent antioxidant capacity (TEAC) in serum of d-galactose-aged mice, while reducing lipofuscin levels, another indicator of cell aging, indicating a potential association with anti-aging activities in a dose dependent manner. This compound had a favourable influence on immune organ indices, and a marked amelioration ability of histopathological hepatic lesions such as necrosis, karyolysis and reduced inflammation and apoptosis in mouse hepatocytes. These results suggest that SHP-1 has strong antioxidant activities and a significant protective effect against oxidative stress or hepatotoxicity induced by d-galactose in mice and it could be developed as a food ingredient or a pharmaceutical to prevent many age-associated diseases such as major depressive disorder and hepatotoxicity. To our knowledge, this is the first report on the antioxidant effects of a novel purified exopolysaccharide derived from Phellinus sp. © 2016 The Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
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