Integrated analysis of long noncoding RNAs and mRNA expression profiles reveals the potential role of lncRNAs in early stage of post-peripheral nerve injury in Sprague-Dawley rats
| Autor: | Yujing Zhang, Zhaowei Zhu, Bo He, Yi Zhang, Yangbin Xu, Shuqia Xu, Xiangxia Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Aging
Phosphatase binding Biology Deep sequencing Rats Sprague-Dawley Peripheral Nerve Injuries medicine Animals peripheral nerve injury Gene Regulatory Networks RNA Messenger long noncoding RNA Gene Expression Profiling Reproducibility of Results bioinformatics Cell Biology Cell cycle Sciatic nerve injury medicine.disease Sciatic Nerve Transmembrane protein Long non-coding RNA Cell biology Gene Ontology Gene Expression Regulation Peripheral nerve injury RNA Long Noncoding Signal transduction Research Paper lncRNA-mRNA network |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.202989 |
| Popis: | The regulatory role of lncRNAs in the early stage post peripheral nerve injury (PNI) is not yet clear. In this study, next-generation sequencing was used to perform deep sequencing on normal sciatic nerves (control) and lesional tissues derived on the 4th (D4) and 7th days (D7) after sciatic nerve injury in rats. Time-point unique differentially expressed lncRNAs (DElncRNAs) were analyzed for functional enrichment. The results showed that 776 DElncRNAs were unique to D4, and their functions were mainly enriched in wound healing, phosphatase binding and MAPK signaling pathways; 317 DElncRNAs were unique to D7, and their functions were mainly enriched in ion transmembrane transporter channel activity; 579 DElncRNAs were shared by these two days, and their functions were mainly enriched in axongenesis, the PI3K-Akt signaling pathway and cell cycle. Furthermore, lncRNA-mRNA interaction networks were constructed in functions or pathways with a high enrichment rate. Finally, 3 mRNAs and 4 lncRNAs in the axongenesis interaction network were selected, and their expression levels were verified by RT-qPCR. This study preliminarily revealed the regulatory role of lncRNAs at different time points in the early stage post PNI, which provides potential targets for basic research and clinical treatment of PNI. |
| Databáze: | OpenAIRE |
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