Chronic sympathetic driven hypertension promotes atherosclerosis by enhancing hematopoiesis
Autor: | Alyce J. Nicholls, Man K.S. Lee, Michael J Kraakman, Andrew J. Murphy, Kristy L. Jackson, Prabhakara R Nagareddy, Jaye Chin-Dusting, Annas Al-Sharea, Ann Maree Jefferis, Olivia D. Cooney, Geoff Head, Danielle L. Michell, Alexandra Whillas, Connie H.Y. Wong, Karen L. Andrews, Gavin W. Lambert, Waled A. Shihata, Camilla Bertuzzo Veiga |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Sympathetic nervous system
Sympathetic Nervous System Biopsy CXCR4 Article 03 medical and health sciences Mice 0302 clinical medicine Bone Marrow Medicine Animals Progenitor cell Endothelial dysfunction Stem Cell Niche Mice Knockout Myelopoiesis business.industry Hematology medicine.disease Atherosclerosis Hematopoietic Stem Cells Immunohistochemistry 3. Good health Extramedullary hematopoiesis Hematopoiesis Haematopoiesis Disease Models Animal medicine.anatomical_structure Phenotype Hypertension Cancer research Bone marrow Disease Susceptibility business Biomarkers 030215 immunology Autonomic Nerve Block Signal Transduction |
Zdroj: | Haematologica |
ISSN: | 1592-8721 0390-6078 |
Popis: | Hypertension is a major, independent risk factor for atherosclerotic cardiovascular disease. However, this pathology can arise through multiple pathways, which could influence vascular disease through distinct mechanisms. An overactive sympathetic nervous system is a dominant pathway that can precipitate in elevated blood pressure. We aimed to determine how the sympathetic nervous system directly promotes atherosclerosis in the setting of hypertension. We used a mouse model of sympathetic nervous system-driven hypertension on the atherosclerotic-prone apolipoprotein E-deficient background. When mice were placed on a western type diet for 16 weeks, we showed the evolution of unstable atherosclerotic lesions. Fortuitously, the changes in lesion composition were independent of endothelial dysfunction, allowing for the discovery of alternative mechanisms. With the use of flow cytometry and bone marrow imaging, we found that sympathetic activation caused deterioration of the hematopoietic stem and progenitor cell niche in the bone marrow, promoting the liberation of these cells into the circulation and extramedullary hematopoiesis in the spleen. Specifically, sympathetic activation reduced the abundance of key hematopoietic stem and progenitor cell niche cells, sinusoidal endothelial cells and osteoblasts. Additionally, sympathetic bone marrow activity prompted neutrophils to secrete proteases to cleave the hematopoietic stem and progenitor cell surface receptor CXCR4. All these effects could be reversed using the β-blocker propranolol during the feeding period. These findings suggest that elevated blood pressure driven by the sympathetic nervous system can influence mechanisms that modulate the hematopoietic system to promote atherosclerosis and contribute to cardiovascular events. |
Databáze: | OpenAIRE |
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