Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus

Autor: Matheus Takemi Muchon Nakatani, Carlos Alexandre Carollo, Fernando de Almeida Borges, Aline Regina Hellmann Carollo, Mariana Green de Freitas, Mário Henrique Conde, Dyego Gonçalves Lino Borges, M. A. Araújo, Adrian Luis Lifschitz, Zelina dos Santos Freire, Juliane Francielle Tutija
Rok vydání: 2020
Předmět:
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 0001-706X
Popis: The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep. Fil: Borges, Dyego Gonçalves Lino. Universidade Federal do Mato Grosso do Sul; Brasil Fil: de Araújo, Marcelo Augusto. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Carollo, Carlos Alexandre. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Hellmann Carollo, Aline Regina. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Conde, Mário Henrique. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Green de Freitas, Mariana. Universidade Federal do Mato Grosso do Sul; Brasil Fil: dos Santos Freire, Zelina. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Francielle Tutija, Juliane. Universidade Federal do Mato Grosso do Sul; Brasil Fil: Takemi Muchon Nakatani, Matheus. Universidade Federal do Mato Grosso do Sul; Brasil Fil: de Almeida Borges, Fernando. Universidade Federal do Mato Grosso do Sul; Brasil
Databáze: OpenAIRE
Externí odkaz: