Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations
| Autor: | Sasa Djordjevic, Florian Sotier, Hauke Clausen-Schaumann, Jun Zhang, Denitsa Docheva, Stefanie Sudhop, Heinz P. Huber, Yasemin Geiger |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Femto 610 Medizin Article law.invention law TJ1-1570 single-cell bioprinting Mechanical engineering and machinery Electrical and Electronic Engineering Jet (fluid) Range (particle radiation) ddc:610 picosecond laser-based bioprinting business.industry Mechanical Engineering femtosecond laser-based bioprinting Pulse duration Laser laser-induced forward transfer (LIFT) Pulse (physics) film-free LIFT tissue engineering Control and Systems Engineering Picosecond Femtosecond Optoelectronics business |
| Zdroj: | Micromachines Volume 12 Issue 10 Micromachines, Vol 12, Iss 1172, p 1172 (2021) |
| Popis: | Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer. |
| Databáze: | OpenAIRE |
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