Gene expression profiling in single cells within tissue
| Autor: | Heidi Buchinsky, Michael J. Donovan, Carlos Cordon-Cardo, Paola Capodieci, Yusuf Jeffers, William L. Gerald, Shailesh M. Shenoy, Jon Edelson, Robert H. Singer |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Context (language use) In situ hybridization Computational biology Biology Biochemistry Article Gene expression Biomarkers Tumor Tumor Cells Cultured Humans Tissue Distribution Multiplex Molecular Biology In Situ Hybridization Fluorescence Paraffin Embedding Gene Expression Profiling Prostatic Neoplasms RNA Cell Biology Molecular biology Neoplasm Proteins Gene expression profiling Tissue sections Fish Biotechnology |
| Zdroj: | Nature Methods. 2:663-665 |
| ISSN: | 1548-7105 1548-7091 |
| Popis: | We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures. |
| Databáze: | OpenAIRE |
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