Interactions of the flavonoid naringenin in the gastrointestinal tract and the influence of glycosylation
| Autor: | Catherine Rice-Evans, Edward S. Debnam, George Chowrimootoo, Kaila Singh Srai, Ruksana Choudhury |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Naringenin
Male Glycosylation Metabolite Biophysics Glucuronidation Administration Oral Pharmacology Biochemistry Intestinal absorption chemistry.chemical_compound medicine Animals Drug Interactions Rats Wistar Molecular Biology Chromatography High Pressure Liquid Flavonoids Gastrointestinal tract food and beverages Cell Biology Small intestine Rats Aglycone medicine.anatomical_structure chemistry Intestinal Absorption Flavanones Injections Intravenous Glucuronide Digestive System |
| Zdroj: | Biochemical and biophysical research communications. 265(2) |
| ISSN: | 0006-291X |
| Popis: | We have studied interactions in the gastrointestinal tract of flavonoids and the influence of glycosylation on their subsequent metabolism by examining the urinary recoveries of the flavonoid naringenin-7-glucoside and its aglycone, in the conscious rat model, after oral and intravenous administration. Absorption studies were also conducted using an in vitro isolated rat jejunum. The results show that ca. 10% of the administered dose of naringenin was recovered after oral dosing, the majority as naringenin glucuronide, whereas, after intravenous administration, the recovery of the glucuronide was ca. 20%. In contrast, after oral dosing of naringenin-7-glucoside, its hydrolysis product naringenin (0.5%) and naringenin glucuronide (12.7%) were detected. After intravenous dosing the majority of that identified in the urine was as the native glucoside. These findings suggest that, via the oral route, the glycoside group is cleaved by an intestinal enzyme prior to glucuronidation within the epithelium. This is substantiated by the urinary elimination of the native glucoside and the lack of detection of glucuronide after intravenous administration. Transport studies with isolated intestine showed that neither unchanged naringenin nor the 7-glucoside was absorbed in significant quantities across the gut wall. The major metabolite detected in both cases was naringenin glucuronide, thus supporting the notion that glucuronidation as well as hydrolysis can occur at the intestinal epithelium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect