Detection of RAGE expression and its application to diabetic wound age estimation
| Autor: | Guang-Hua Ye, Ke-Zhi Lin, Yang Chen, Xing-Biao Li, Xin-Yi Ji, Lin-Sheng Yu, Miao-Wu Dong, Xiang-Ping Feng, Jun-Ge Han, Yan-Yan Fan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Time Factors Blotting Western Receptor for Advanced Glycation End Products Fluorescent Antibody Technique Inflammation Pathology and Forensic Medicine RAGE (receptor) Diabetes Mellitus Experimental 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Glycation Diabetes mellitus medicine Animals 030216 legal & forensic medicine Receptor Skin Wound Healing integumentary system business.industry medicine.disease Immunohistochemistry Up-Regulation Blot Mice Inbred C57BL 030104 developmental biology medicine.symptom business Biomarkers |
| Zdroj: | International journal of legal medicine. 131(3) |
| ISSN: | 1437-1596 |
| Popis: | With the prevalence of diabetes, it is becoming important to analyze the diabetic wound age in forensic practice. The present study investigated the time-dependent expression of receptor for advanced glycation end products (RAGE) during diabetic wound healing in mice and its applicability to wound age determination by immunohistochemistry, double immunofluorescence, and Western blotting. After an incision was created in genetically diabetic db/db mice and control mice, mice were killed at posttraumatic intervals ranging from 6 h to 14 days, followed by the sampling of wound margin. Compared with control mice, diabetic mice showed the delayed wound healing. In control and diabetic wound specimens, RAGE immunoreactivity was observed in a small number of polymorphonuclear cells (PMNs), a number of macrophages, and fibroblasts. Morphometrically, the positive ratios of RAGE in macrophages or fibroblasts considerably increased in diabetic wounds during late repair, which exceeded 60% at 7 and 10 days post-injury. There were no control wound specimens to show a ratio of >60% in macrophages or fibroblasts. By Western blotting analysis, the ratios of RAGE to GAPDH were >1.4 in all diabetic wound samples from 7 to 10 days post-injury, which were >1.8 at 10 days after injury. By comparison, no control wound specimens indicated a ratio of >1.4. In conclusion, the expression of RAGE is upregulated and temporally distributed in macrophages and fibroblasts during diabetic wound healing, which might be closely involved in prolonged inflammation and deficient healing. Moreover, RAGE is promising as a useful marker for diabetic wound age determination. |
| Databáze: | OpenAIRE |
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