Non-covalent Monolayer-Piercing Anchoring of Lipophilic Nucleic Acids
| Autor: | Eli Flaxer, Moria Kwiat, Jan Willem de Vries, Fernando Patolsky, Yoni Engel, Roey Elnathan, Andreas Herrmann, Artium Khatchtourints, Minseok Kwak, Alexander Pevzner, Amir Lichtenstein, Larisa Burstein |
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| Přispěvatelé: | Polymer Chemistry and Bioengineering, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Kwiat, Moria, Elnathan, Roey, Kwak, Minseok, De, Vries Jan, Pevzner, Alexander, Engel, Yoni, Burstein, Larisa, Khatchtourints, Artium, Lichtenstein, Amir, Flaxer, Eli, Hermann, Andreas, Patolsky, Fernando |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Silicon Hot Temperature DEVICES Surface Properties Supramolecular chemistry Nanotechnology Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Catalysis Contact angle chemistry.chemical_compound Colloid and Surface Chemistry LABEL-FREE DETECTION Amphiphile Monolayer BLOCK-COPOLYMER MICELLES SENSORS Bifunctional AMPHIPHILES Electrodes chemistry.chemical_classification Base Sequence ELECTRICAL DETECTION Biomolecule DNA HYBRIDIZATION Nucleic Acid Hybridization DNA General Chemistry Quartz crystal microbalance 021001 nanoscience & nanotechnology ALKANETHIOL MONOLAYERS 0104 chemical sciences nucleic acids Oligodeoxyribonucleotides Semiconductors chemistry Nucleic Acid Conformation Glass Gold FIELD-EFFECT TRANSISTORS 0210 nano-technology Hydrophobic and Hydrophilic Interactions Biosensor SILICON NANOWIRES |
| Zdroj: | Journal of the American Chemical Society Journal of the American Chemical Society, 134(1), 280-292. AMER CHEMICAL SOC |
| ISSN: | 0002-7863 |
| Popis: | Functional interfaces of biomolecules and inorganic substrates like semiconductor materials are of utmost importance for the development of highly sensitive biosensors and microarray technology. However, there is still a lot of room for improving the techniques for immobilization of biomolecules, in particular nucleic acids and proteins. Conventional anchoring strategies rely on attaching biomacromolecules via complementary functional groups, appropriate bifunctional linker molecules, or non-covalent immobilization via electrostatic interactions. In this work, we demonstrate a facile, new, and general method for the reversible non-covalent attachment of amphiphilic DNA probes containing hydrophobic units attached to the nucleobases (lipid-DNA) onto SAM-modified gold electrodes, silicon semiconductor surfaces, and glass substrates. We show the anchoring of well-defined amounts of lipid-DNA onto the surface by insertion of their lipid tails into the hydrophobic monolayer structure. The surface coverage of DNA molecules can be conveniently controlled by modulating the initial concentration and incubation time. Further control over the DNA layer is afforded by the additional external stimulus of temperature. Heating the DNA-modified surfaces at temperatures > 80 degrees C leads to the release of the lipid-DNA structures from the surface without harming the integrity of the hydrophobic SAMs. These supramolecular DNA layers can be further tuned by anchoring onto a mixed SAM containing hydrophobic molecules of different lengths, rather than a homogeneous SAM. Immobilization of lipid-DNA on such SAMs has revealed that the surface density of DNA probes is highly dependent on the composition of the surface layer and the structure of the lipid-DNA. The formation of the lipid-DNA sensing layers was monitored and characterized by numerous techniques including X-ray photoelectron spectroscopy, quartz crystal microbalance, ellipsometry, contact angle measurements, atomic force microscopy, and confocal fluorescence imaging. Finally, this new DNA modification strategy was applied for the sensing of target DNAs using silicon-nanowire field-effect transistor device arrays, showing a high degree of specificity toward the complementary DNA target, as well as single-base mismatch selectivity. |
| Databáze: | OpenAIRE |
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